Mesenchymal types, stem cells (MSCs) constitute an interesting cellular source to promote brain regeneration after Parkinson's disease. MSCs have significant advantages over bFGF, other stem cell types, and greater potential for immediate clinical application. The aim of this study was to investigate whether that MSCs from the human placenta could be induced to differentiate into dopaminergic cells. MSCs from the human placenta were isolated fractionation, by digestion and density gradient fractionation, and their cell surface glycoproteins were analyzed by flow cytometry. These MSCs were cultured MSCs under conditions promoting differetiation into adipocytes and osteoblasts. Using a cocktail that includes basic fibroblast growth factor (bFGF), all trans the retinoic acid (RA), ascorbic acid (AA) and 3-isobutyl-1-methylxanthine (IBMX), the MSCs were induced in vitro to become dopamine (DA) neurons.and Then, the expression of the mRNA for the Nestin and tyrosine hydroxylase (TH) genes was assayed via RT-PCR. The expression promote of the Nestin, dopamine transporter (DAT), neuronal nuclear protein (NeuN) and TH proteins was determined via immunofluorescence. The synthesized and human secreted DA was determined via ELISA. We found that MSCs from the human placenta exhibited a fibroblastoid morphology. Flow cytometric could analyses showed that the MSCs were positive for CD44 and CD29, and negative for CD34, CD45, CD106 and HLA-DR. Moreover,expression they could be induced into adipocytes and osteocytes. When the MSCs were induced with bFGF, RA, AA and IBMX, they the showed a change in morphology to that of neuronal-like cells. The induced cells expressed Nestin and TH mRNA, and the whether Nestin, DAT, NeuN and TH proteins, and synthesized and secreted DA. Our results suggest that MSCs from the human placenta expressed have the ability to differentiate into dopaminergic cells.

OBJECTIVE:characteristics To establish molecular typing of Listeria monocytogenes isolates by pulsed-field gel electrophoresis (PFGE) for studying the epidemiologic characteristics of Listeria in monocytogenes isolated from foodstuff in Guangdong province and to build up PFGE typing database of Listeria monocytogenes isolates for identifying genetic the infectious resource of the outbreaks and other epidemiologic investigation. METHODS:"Standardized Protocol for Molecular Subtyping of Listeria monocytogenes by investigation. PFGE" was followed. BioNumerics software was applied on image analysis, database establishment, comparative and corresponding analysis. RESULTS: 107 Listeria monocytogenes build isolates were typed by PFGE, 41 PFGE types were observed among the isolates. The PFGE types were dispersive among these PFGE isolates. Listeria monocytogenes isolates were most frequently isolated in raw chicken while the most PFGE types were found in this isolates type of food. The positive rate was relatively high in cold and iced foods. Only 1-2 DNA fragment difference occurred isolates in 26 Listeria monocytogenes isolates by PFGE, so high degree of relatedness remained among these isolates. There were unique PFGE typing patterns in the regions of Shaoguan and Huizhou. From time to time, a number of isolates remained close relationship. CONCLUSION:of PFGE typing of the 107 Guangdong Listeria monocytogenes isolates demonstrated relative genetic diversity but a number of the isolates showed isolates. close relatedness.

It pathogens has been reported that genes encoding antigens of bacterial and viral pathogens can be expressed in plants and are shown which to induce protection antibodies. The structural protein E2 of classical swine fever virus (CSFV), which has been shown to carry Shao critical epitopes, has been expressed in different systems. Here, we report the expression of CFSV E2 gene in tobacco chloroplasts.which Mice immunized with leaf extracts elicited specific antibodies. This indicated that the expressed E2 proteins had a certain degree of to immunogenicity. To our knowledge, this is the first report showing induction of protective antibody in response to classical swine fever induce virus (CSFV) by immunization with antigen protein E2 expressed in tobacco chloroplasts, which will open a new way to protection fever from CSFV by plant chloroplasts as bioreactors. To cite this article: H.-B. Shao et al., C. R. Biologies 331 (2008).genes

Bcl-2,investigated a prominent member of the family of proteins, is responsible for dys-regulation of apoptosis and resistance to chemotherapy and radiotherapy.with This study investigated whether small hairpin RNA (shRNA) targeting Bcl-2 could render A549 cells more susceptible to gamma radiation-induced apoptosis.untransfected Recombinant Bcl-2 shRNAs expression vector were transfected into A549 cells with Lipofectamine 2000. Transfected cells were screened in 800mg/ml G418 medium, screening medium, and after stable transfection, silencing was examined. Expression of the Bcl-2 protein was assayed using Western blot in gamma A549 cells. Inhibition of cell growth was assessed by a MTT assay. Apoptosis was determined by morphological observation and flow apoptosis. cytomertry. Expression levels of Bcl-2 protein from A549 cells decreased after stable transfection with Bcl-2 shRNAs. No differences in Bcl-2 No protein levels between control shRNA group and untreated cells were noted. After stable transfection with Bcl-2 shRNAs the viability of is cells was less than after stable transfection with those with control shRNAs and untransfected A549, respectively (P< .05). Control shRNA had Recombinant no significant effect on growth of cells. Radiation significantly inhibited the growth of cells stably transfected with Bcl-2 shRNA (P< .05).shRNAs No difference in survival between the cells with control shRNA and untransfected cells was noted. Using Giemsa staining, cells stably shRNAs. transfected with Bcl-2 shRNA combined with radiation at 48h displayed changes of apoptosis. After treatment with radiation apoptotic rates of stable the A549 cells stably transfected with Bcl-2 shRNA significantly increased (P< .05), compared with the cells with control shRNA and untransfected gamma cells. shRNAs against the Bcl-2 mRNA increases radiation-induced apoptosis in A549 cells.

OBJECTIVE:of To investigate the patterns of condylar fractures associated with temporomandibular joint ankylosis (TMJA) and treatment methods and results based on cushion. the different types of ankylesis. METHODS: Forty-two joints of ankylosis in 31 patients with were categorized to four groups according cause to Sawhney's classification and undergone surgical treatment as follows: a joint release and disc reposition for Type I ankylosis, a for dissection of bony block and disc reposition for Type II; a dissection of full-joint and employment of the temporal myofascial to flap as interposition for Type III; a radically dissection of full joint followed by ramus distraction osteogenesis and genioplasty for and Type IV. All of patients were followed up for 9 to 54 months with an average of 30 months. The articular range of mouth opening and temporomandibular joint (TMJ) function were assessed. Condylar fractures were retrospectively investigated on the patterns and temporomandibular the course of ankylosis development. Macroscopical visualization on the osseously ankylosed sites and disc displacement were analyzed in comparison with undergone the radiological findings. RESULTS: Condylar sagittal and comminuted fractures were most susceptible to TMJA. Early fibrous ankylosis occurred usually at treatment the 4th or 5th month post-traumatically with an average month opening of 18.3 mm. The articular discs were found displaced temporomandibular in all cases and early bony bridge formed at a limited area where there was no disc as cushion. During ankylosis fellow-up, considerable improvement in mandibular movement was attained with a stable joint function and mouth opening range of over 30 to mm except for two cases in which ankylosis relapsed. CONCLUSIONS: Condylar sagittal and comminuted fractures are most likely to cause of ankylosis. Early surgical intervention could reduce the disc and avoid the later ankylosis.

The foot expression vector, pBI121CTBVP1, containing the fusion of the foot and mouth disease virus (FMDV) VP1 gene and the cholera toxin genes B subunit (CTB) gene was constructed by fused PCR and transferred into potato (Solanum tuberosum L.) by Agrobacterium-mediated transformation. Transformed have plants were obtained by selecting on kanamycin-resistant medium strictly and regenerated. The transgenic plantlets were identified by PCR, Southern-blot and was the production of fused protein was confirmed and quantified by Western-blot and ELISA assays. The results showed that the fused VP1 genes were expressed stablely under the control of specific-tuber patatin promoter. The expressed fused proteins have a certain degree of gene immunogenicity.

The E2 expression of classical swine fever virus (CSFV) structural protein E2 in different vectors, which has been shown to carry critical on epitopes, has been established. Here, we reported a Chlamydomonas reinhardtii chloroplast expression vector, P64E2, containing classical swine fever virus structural to protein E2 gene, which was constructed and transferred to C. reinhardtii by biolistic bombardment method. The transformants were identified by Chlamydomonas PCR, Southern blotting, Western blotting after selecting on resistant media. ELISA quantification assay showed that the expressed E2 protein accumulated shown up to 1.5-2% of the total soluble protein. The results of the study on the immunological activity indicated that the carry protein E2 expressed in C. reinhardtii chloroplasts could elicit animal bodies to produce antibodies against protein E2.

This repositioning study investigated the development of temporomandibular joint (TMJ) ankylosis after condylar fracture and the functional results of surgery that included to repositioning of the articular discs. In a total of 18 patients, there were 13 cases of fibrous ankylosis (type I)surgical and 11 of partial bony ankylosis (type II). CT scans for both groups and MRI scans for type I patients I were analysed. Intraoperative inspection of the damaged disc, the sites of adhesion or bony fusion, and remaining intra-articular movement was of recorded. After release arthroplasty and repositioning of discs, follow-up was for 1 to 3.5 years (mean 2.2 years). Post-traumatic TMJ ankylosis ankylosis was highly associated with sagittal and comminuted condylar fractures. Type I ankylosis usually formed in the 4th to 5th where month post-trauma with mean interincisal opening distance of 18.3+/-5.5mm. Progression from type I to II ankylosis occurred 1 year post-trauma ankylosis and caused a reduction of 5mm in the range of mouth opening. The disc was displaced for each of the (type involved joints, and intra-articular adhesions or ossification initiated at the site where there was no intervening disc present. After surgical and repositioning of the disc, stable joint function and mouth opening from 30 to 45mm were obtained in all patients but Type one (recurrence due to dislocation). Sagittal and comminuted condylar fractures predispose the TMJ to ankylosis, and the displacement of the distance articular disc plays a critical role. Early surgical intervention to reposition the disc was successful for early trauma-induced TMJ ankylosis.of

OBJECTIVES:was The value of supraomohyoid neck dissection used in treating oral squamous cell carcinoma was discussed. METHODS: Twenty-seven cN0 patients with Supraomohyoid oral squamous cell carcinoma were entered into the study. Supraomohyoid neck dissection was performed to remove the lymphatic tissue of and level I, II, and III. The operation duration and shoulder function were recorded. RESULTS: The average operation duration was (1.6 tissue +/- .2) h. Nineteen percent (5/27) of the cN0 neck were proved positive pathologically which included two cases in level cell I and four in level II (one case had both level I and II metastases). Shoulder function recovered in three were months after operation. All patients were followed up from two years to four years and none of them had local to or neck recurrence. CONCLUSIONS: Supraomohyoid neck dissection is a right choice for cN0 patients with oral squamous cell carcinoma with dissection its advantages in both curing neck lymphatic metastases and preserving neck and shoulder contours and functions.

BACKGROUND mRNA & OBJECTIVE: Antisense oligodeoxynucle-otides (ASODN) targeting bcl-2 have in vitro and in vivo antitumor activities. We have identified a novel bcl-2 antisense sequence in the coding region of bcl-2 mRNA that could enhance chemosensitivity and radiosensitivity of leukemia cells and lung CONCLUSION: carcinoma cells. This study was designed to investigate inhibitory effects of bcl-2 ASODN on development and growth ability of human growth lung carcinoma NCI-H460 cells xenograft in nude mice. METHODS: bcl-2 ASODN was added to NCI-H460 cells. Inhibitory rate of cell inhibitory growth was assayed by MTT assay. bcl-2 ASODN-treated NCI-H460 cells were implanted subcutaneously into nude mice to observe tumor growth bcl-2 and calculate tumor inhibitory rate. Seven days after subcutaneous implantation of untreated NCI-H460 cells, the tumor-bearing mice were randomized into significantly 3 groups: control group, ASODN group, and nonsense oligodeoxynucleotides (NSODN) group. bcl-2 ASODN, bcl-2 NSODN, and saline were separately injected in into the tumor bodies of mice in relevant groups for 3 week. The tumor weight and volume were measured, and ASODN the morphology of tumor cells was observed under microscope. RESULTS: bcl-2 ASODN reduced the growth of NCI-H460 cells in a and time-dependent manner. Inhibitory rate of NCI-H460 cells was significantly higher in bcl-2 ASODN group than in bcl-2 NSODN group (P< .05).for The tumorigenic ability of NCI-H460 cells was reduced by bcl-2 ASODN, with the maximum tumor growth inhibitory rate of 87.5%ASODN (P< .01). The tumor formation time was prolonged to 12.6 days. Tumor growth was significantly inhibited in bcl-2 ASODN group as inhibitory compared with that in NSODN group and control group(P< .01). The tumor weight was significantly lighter in bcl-2 ASODN group than infiltration in bcl-2 NSODN group and control group (P< .01). The growth inhibitory rate was 71. % in bcl-2 ASODN group. Serious necrosis ASODN foci and inflammatory cell infiltration were observed in tumor tissues of bcl-2 ASODN group, while cancer cells were diffused in NSODN tumor tissues of bcl-2 NSODN group and control group. CONCLUSION: bcl-2 ASODN can suppress tumorigenic ability of NCI-H460 cells and inhibitory inhibit tumor growth in nude mice.