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Latest Paper:
B N Nagpal,
Rekha Saxena,
Aruna Srivastava,
Neeru Singh,
S K Ghosh,
S K Sharma,
Ashwani Kumar,
Hemant Kumar,
Alok Suman Sharma,
S K Chand,
V P Ojha,
S S Mohanty,
A K Mohanty,
R K Dasgupta,
G P S Dhillon,
A P Dash
National Institute of Malaria Research (ICMR), New Delhi, India.
Background & objectives: A retrospective study on chikungunya outbreak in India in five States viz. Delhi, Madhya Pradesh, Orissa, Maharashtra and Kerala was conducted in 2007-2008 to know the distribution and determinants of chikungunya fever outbreak in India. Methods: On the basis of high and low incidence of chikungunya fever, two districts from each State and two wards from the selected district were taken for random selection of 1000 households from 10 districts and 5 States. Semi-structured questionnaires were administered to individuals, patients, qualified health professionals and to stakeholders for collecting information. Results: The educational background and occupation of the respondents showed variations across the study States. Only in high incidence ward of Maharashtra, water storage period for 3-6 days and emptying, drying of water containers on weekly basis was noted. The study through knowledge, attitude, belief, practice (KABP) obtained individual's perception of chikungunya fever, its prevention and control. Patients' expenditure on treatment was mainly recorded less than Rs 500 across study States. Health facility survey obtained an overview of the capacity of local health facilities. Stakeholders' perception regarding chikungunya fever was also noted. Interpretation & Conclusions: The study revealed differences in awareness of chikungunya, cause of the disease, vector responsible, mode of transmission, biting time and elimination of breeding of mosquitoes statistically significant among high and low incidence wards of all the States. Expenditure on treatment was independent of economically active status and loss of man-days across all the States. Education and occupation did not have any relation with emptying/drying of water containers in high incidence wards. Strengthening of surveillance, information, education and communication (IEC) activities along with case management facilities may be provided by the State health department for prevention of chikungunya outbreaks in future. Stakeholders should be more involved in outbreak management and future planning.
S Chatrchyan,
V Khachatryan,
A M Sirunyan,
A Tumasyan,
W Adam,
T Bergauer,
M Dragicevic,
J Erö,
C Fabjan,
M Friedl,
R Frühwirth,
V M Ghete,
J Hammer,
M Hoch,
N Hörmann,
J Hrubec,
M Jeitler,
W Kiesenhofer,
M Krammer,
D Liko,
I Mikulec,
M Pernicka,
B Rahbaran,
C Rohringer,
H Rohringer,
R Schöfbeck,
J Strauss,
A Taurok,
F Teischinger,
P Wagner,
W Waltenberger,
G Walzel,
E Widl,
C-E Wulz,
V Mossolov,
N Shumeiko,
J Suarez Gonzalez,
S Bansal,
L Benucci,
T Cornelis,
E A De Wolf,
X Janssen,
S Luyckx,
T Maes,
L Mucibello,
S Ochesanu,
B Roland,
R Rougny,
M Selvaggi,
H Van Haevermaet,
P Van Mechelen,
N Van Remortel,
A Van Spilbeeck,
F Blekman,
S Blyweert,
J D'Hondt,
R Gonzalez Suarez,
A Kalogeropoulos,
M Maes,
A Olbrechts,
W Van Doninck,
P Van Mulders,
G P Van Onsem,
I Villella,
O Charaf,
B Clerbaux,
G De Lentdecker,
V Dero,
A P R Gay,
G H Hammad,
T Hreus,
A Léonard,
P E Marage,
L Thomas,
C Vander Velde,
P Vanlaer,
J Wickens,
V Adler,
K Beernaert,
A Cimmino,
S Costantini,
G Garcia,
M Grunewald,
B Klein,
J Lellouch,
A Marinov,
J McCartin,
A A Ocampo Rios,
D Ryckbosch,
N Strobbe,
F Thyssen,
M Tytgat,
L Vanelderen,
P Verwilligen,
S Walsh,
E Yazgan,
N Zaganidis,
S Basegmez,
G Bruno,
L Ceard,
J De Favereau De Jeneret,
C Delaere,
T du Pree,
D Favart,
L Forthomme,
A Giammanco,
G Grégoire,
J Hollar,
V Lemaitre,
J Liao,
O Militaru,
C Nuttens,
D Pagano,
A Pin,
K Piotrzkowski,
N Schul,
N Beliy,
T Caebergs,
E Daubie,
G A Alves,
M Correa Martins Junior,
D De Jesus Damiao,
T Martins,
M E Pol,
M H G Souza,
W L Aldá Júnior,
W Carvalho,
A Custódio,
E M Da Costa,
C De Oliveira Martins,
S Fonseca De Souza,
D Matos Figueiredo,
L Mundim,
H Nogima,
V Oguri,
W L Prado Da Silva,
A Santoro,
S M Silva Do Amaral,
L Soares Jorge,
A Sznajder,
T S Anjos,
C A Bernardes,
F A Dias,
T R Fernandez Perez Tomei,
E M Gregores,
C Lagana,
F Marinho,
P G Mercadante,
S F Novaes,
Sandra S Padula,
V Genchev,
P Iaydjiev,
S Piperov,
M Rodozov,
S Stoykova,
G Sultanov,
V Tcholakov,
R Trayanov,
M Vutova,
A Dimitrov,
R Hadjiiska,
A Karadzhinova,
V Kozhuharov,
L Litov,
B Pavlov,
P Petkov,
J G Bian,
G M Chen,
H S Chen,
C H Jiang,
D Liang,
S Liang,
X Meng,
J Tao,
J Wang,
X Wang,
Z Wang,
H Xiao,
M Xu,
J Zang,
Z Zhang,
C Asawatangtrakuldee,
Y Ban,
S Guo,
Y Guo,
W Li,
S Liu,
Y Mao,
S J Qian,
H Teng,
S Wang,
B Zhu,
W Zou,
A Cabrera,
B Gomez Moreno,
A F Osorio Oliveros,
J C Sanabria,
N Godinovic,
D Lelas,
R Plestina,
D Polic,
I Puljak,
Z Antunovic,
M Dzelalija,
M Kovac,
V Brigljevic,
S Duric,
K Kadija,
J Luetic,
S Morovic,
A Attikis,
M Galanti,
J Mousa,
C Nicolaou,
F Ptochos,
P A Razis,
M Finger,
M Finger Jr,
Y Assran,
A Ellithi Kamel,
S Khalil,
M A Mahmoud,
A Radi,
A Hektor,
M Kadastik,
M Müntel,
M Raidal,
L Rebane,
A Tiko,
V Azzolini,
P Eerola,
G Fedi,
M Voutilainen,
S Czellar,
J Härkönen,
A Heikkinen,
V Karimäki,
R Kinnunen,
M J Kortelainen,
T Lampén,
K Lassila-Perini,
S Lehti,
T Lindén,
P Luukka,
T Mäenpää,
T Peltola,
E Tuominen,
J Tuominiemi,
E Tuovinen,
D Ungaro,
L Wendland,
K Banzuzi,
A Korpela,
T Tuuva,
D Sillou,
M Besancon,
S Choudhury,
M Dejardin,
D Denegri,
B Fabbro,
J L Faure,
F Ferri,
S Ganjour,
A Givernaud,
P Gras,
G Hamel de Monchenault,
P Jarry,
E Locci,
J Malcles,
L Millischer,
J Rander,
A Rosowsky,
I Shreyber,
M Titov,
S Baffioni,
F Beaudette,
L Benhabib,
L Bianchini,
M Bluj,
C Broutin,
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C Charlot,
N Daci,
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L Dobrzynski,
S Elgammal,
R Granier de Cassagnac,
M Haguenauer,
P Miné,
C Mironov,
C Ochando,
P Paganini,
D Sabes,
R Salerno,
Y Sirois,
C Thiebaux,
C Veelken,
A Zabi,
J-L Agram,
J Andrea,
D Bloch,
D Bodin,
J-M Brom,
M Cardaci,
E C Chabert,
C Collard,
E Conte,
F Drouhin,
C Ferro,
J-C Fontaine,
D Gelé,
U Goerlach,
P Juillot,
M Karim,
A-C Le Bihan,
P Van Hove,
F Fassi,
D Mercier,
C Baty,
S Beauceron,
N Beaupere,
M Bedjidian,
O Bondu,
G Boudoul,
D Boumediene,
H Brun,
J Chasserat,
R Chierici,
D Contardo,
P Depasse,
H El Mamouni,
A Falkiewicz,
J Fay,
S Gascon,
M Gouzevitch,
B Ille,
T Kurca,
T Le Grand,
M Lethuillier,
L Mirabito,
S Perries,
V Sordini,
S Tosi,
Y Tschudi,
P Verdier,
S Viret,
D Lomidze,
G Anagnostou,
S Beranek,
M Edelhoff,
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A Ostapchuk,
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C Magass,
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W Behrenhoff,
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A Bethani,
K Borras,
A Burgmeier,
A Cakir,
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A Campbell,
E Castro,
D Dammann,
G Eckerlin,
D Eckstein,
A Flossdorf,
G Flucke,
A Geiser,
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M Kasemann,
P Katsas,
C Kleinwort,
H Kluge,
A Knutsson,
M Krämer,
D Krücker,
E Kuznetsova,
W Lange,
W Lohmann,
B Lutz,
R Mankel,
I Marfin,
M Marienfeld,
I-A Melzer-Pellmann,
A B Meyer,
J Mnich,
A Mussgiller,
S Naumann-Emme,
J Olzem,
A Petrukhin,
D Pitzl,
A Raspereza,
P M Ribeiro Cipriano,
M Rosin,
J Salfeld-Nebgen,
R Schmidt,
T Schoerner-Sadenius,
N Sen,
A Spiridonov,
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J Tomaszewska,
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C Wissing,
C Autermann,
V Blobel,
S Bobrovskyi,
J Draeger,
H Enderle,
J Erfle,
U Gebbert,
M Görner,
T Hermanns,
R S Höing,
K Kaschube,
G Kaussen,
H Kirschenmann,
R Klanner,
J Lange,
B Mura,
F Nowak,
N Pietsch,
C Sander,
H Schettler,
P Schleper,
E Schlieckau,
A Schmidt,
M Schröder,
T Schum,
H Stadie,
G Steinbrück,
J Thomsen,
C Barth,
J Berger,
T Chwalek,
W De Boer,
A Dierlamm,
G Dirkes,
M Feindt,
J Gruschke,
M Guthoff,
C Hackstein,
F Hartmann,
M Heinrich,
H Held,
K H Hoffmann,
S Honc,
I Katkov,
J R Komaragiri,
T Kuhr,
D Martschei,
S Mueller,
Th Müller,
M Niegel,
A Nürnberg,
O Oberst,
A Oehler,
J Ott,
T Peiffer,
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K Rabbertz,
F Ratnikov,
N Ratnikova,
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S Röcker,
C Saout,
A Scheurer,
P Schieferdecker,
F-P Schilling,
M Schmanau,
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F M Stober,
D Troendle,
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T Weiler,
M Zeise,
E B Ziebarth,
G Daskalakis,
T Geralis,
S Kesisoglou,
A Kyriakis,
D Loukas,
I Manolakos,
A Markou,
C Markou,
C Mavrommatis,
E Ntomari,
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A Panagiotou,
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F A Triantis,
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K Krajczar,
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B Ujvari,
S B Beri,
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N Dhingra,
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K Ranjan,
V Sharma,
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S Banerjee,
S Bhattacharya,
S Dutta,
B Gomber,
S Jain,
R Khurana,
S Sarkar,
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D Dutta,
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V Kumar,
A K Mohanty,
L M Pant,
P Shukla,
T Aziz,
S Ganguly,
M Guchait,
A Gurtu,
M Maity,
G Majumder,
K Mazumdar,
G B Mohanty,
B Parida,
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S M Etesami,
A Fahim,
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A Jafari,
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A Mohammadi,
M Mohammadi Najafabadi,
S Paktinat Mehdiabadi,
B Safarzadeh,
M Zeinali,
M Abbrescia,
L Barbone,
C Calabria,
S S Chhibra,
A Colaleo,
D Creanza,
N De Filippis,
M De Palma,
L Fiore,
G Iaselli,
L Lusito,
G Maggi,
M Maggi,
N Manna,
B Marangelli,
S My,
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N Pacifico,
A Pompili,
G Pugliese,
F Romano,
G Selvaggi,
L Silvestris,
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S Tupputi,
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G Abbiendi,
G Barozzi,
A C Benvenuti,
D Bonacorsi,
S Braibant-Giacomelli,
L Brigliadori,
P Capiluppi,
A Castro,
F R Cavallo,
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F Fabbri,
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F L Navarria,
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A Tropiano,
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D Piccolo,
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A Benaglia,
F De Guio,
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S Gennai,
A Ghezzi,
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R A Manzoni,
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C A Carrillo Montoya,
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L Lista,
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N Bacchetta,
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D Bisello,
A Branca,
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P Checchia,
T Dorigo,
U Dosselli,
F Fanzago,
F Gasparini,
U Gasparini,
A Gozzelino,
K Kanishchev,
S Lacaprara,
I Lazzizzera,
M Loreti,
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A T Meneguzzo,
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S P Ratti,
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A Nappi,
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T Boccali,
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F Fiori,
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A Giassi,
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A Messineo,
F Palla,
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A Rizzi,
A T Serban,
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P G Verdini,
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F Pandolfi,
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S Rahatlou,
M Sigamani,
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N Amapane,
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C Biino,
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E Migliore,
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N Pastrone,
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S Belforte,
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G Della Ricca,
B Gobbo,
M Marone,
D Montanino,
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S G Heo,
S K Nam,
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J Chung,
D H Kim,
G N Kim,
J E Kim,
D J Kong,
H Park,
S R Ro,
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H Y Jo,
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D Gyun,
B Hong,
M Jo,
H Kim,
T J Kim,
K S Lee,
D H Moon,
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E Seo,
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J H Kim,
C Park,
I C Park,
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J Goh,
M S Kim,
B Lee,
J Lee,
S Lee,
H Seo,
I Yu,
M J Bilinskas,
I Grigelionis,
M Janulis,
H Castilla-Valdez,
E De La Cruz-Burelo,
I Heredia-de La Cruz,
R Lopez-Fernandez,
R Magaña Villalba,
J Martínez-Ortega,
A Sánchez-Hernández,
L M Villasenor-Cendejas,
S Carrillo Moreno,
F Vazquez Valencia,
H A Salazar Ibarguen,
E Casimiro Linares,
A Morelos Pineda,
M A Reyes-Santos,
D Krofcheck,
A J Bell,
P H Butler,
R Doesburg,
S Reucroft,
H Silverwood,
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M I Asghar,
H R Hoorani,
S Khalid,
W A Khan,
T Khurshid,
S Qazi,
M A Shah,
M Shoaib,
G Brona,
M Cwiok,
W Dominik,
K Doroba,
A Kalinowski,
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J Krolikowski,
H Bialkowska,
B Boimska,
T Frueboes,
R Gokieli,
M Górski,
M Kazana,
K Nawrocki,
K Romanowska-Rybinska,
M Szleper,
G Wrochna,
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N Almeida,
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A David,
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P G Ferreira Parracho,
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P Musella,
A Nayak,
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P Q Ribeiro,
J Seixas,
J Varela,
P Vischia,
I Belotelov,
I Golutvin,
N Gorbounov,
I Gramenitski,
A Kamenev,
V Karjavin,
V Konoplyanikov,
V Korenkov,
G Kozlov,
A Lanev,
P Moisenz,
V Palichik,
V Perelygin,
M Savina,
S Shmatov,
S Vasil'ev,
A Zarubin,
S Evstyukhin,
V Golovtsov,
Y Ivanov,
V Kim,
P Levchenko,
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V Oreshkin,
I Smirnov,
V Sulimov,
L Uvarov,
S Vavilov,
A Vorobyev,
An Vorobyev,
Yu Andreev,
A Dermenev,
S Gninenko,
N Golubev,
M Kirsanov,
N Krasnikov,
V Matveev,
A Pashenkov,
A Toropin,
S Troitsky,
V Epshteyn,
M Erofeeva,
V Gavrilov,
M Kossov,
A Krokhotin,
N Lychkovskaya,
V Popov,
G Safronov,
S Semenov,
V Stolin,
E Vlasov,
A Zhokin,
A Belyaev,
E Boos,
M Dubinin,
L Dudko,
A Ershov,
A Gribushin,
O Kodolova,
I Lokhtin,
A Markina,
S Obraztsov,
M Perfilov,
S Petrushanko,
L Sarycheva,
V Savrin,
A Snigirev,
V Andreev,
M Azarkin,
I Dremin,
M Kirakosyan,
A Leonidov,
G Mesyats,
S V Rusakov,
A Vinogradov,
I Azhgirey,
I Bayshev,
S Bitioukov,
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A Mohapatra,
I Ojalvo,
G A Pierro,
I Ross,
A Savin,
W H Smith,
J Swanson
Yerevan Physics Institute, Yerevan, Armenia.
A search for a Higgs boson in the four-lepton decay channel H→ZZ, with each Z boson decaying to an electron or muon pair, is reported. The search covers Higgs boson mass hypotheses in the range of 110<m_{H}<600 GeV. The analysis uses data corresponding to an integrated luminosity of 4.7 fb^{-1} recorded by the CMS detector in pp collisions at sqrt[s]=7 TeV from the LHC. Seventy-two events are observed with four-lepton invariant mass m_{4ℓ}>100 GeV (with 13 below 160 GeV), while 67.1±6.0 (9.5±1.3) events are expected from background. The four-lepton mass distribution is consistent with the expectation of standard model background production of ZZ pairs. Upper limits at 95% confidence level exclude the standard model Higgs boson in the ranges of 134-158 GeV, 180-305 GeV, and 340-465 GeV. Small excesses of events are observed around masses of 119, 126, and 320 GeV, making the observed limits weaker than expected in the absence of a signal.
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Yerevan Physics Institute, Yerevan, Armenia.
A search for signatures of extra spatial dimensions in the diphoton invariant-mass spectrum has been performed with the CMS detector at the LHC. No excess of events above the standard model expectation is observed using a data sample collected in proton-proton collisions at sqrt[s]=7 TeV corresponding to an integrated luminosity of 2.2 fb^{-1}. In the context of the large-extra-dimensions model, lower limits are set on the effective Planck scale in the range of 2.3-3.8 TeV at the 95% confidence level. These limits are the most restrictive bounds on virtual-graviton exchange to date. The most restrictive lower limits to date are also set on the mass of the first graviton excitation in the Randall-Sundrum model in the range of 0.86-1.84 TeV, for values of the associated coupling parameter between 0.01 and 0.10.
Department of Physics, Pondicherry University, Pondicherry 605014, India.
Electrospinning is the most versatile technology in use today, for the generation of polymer nanoscale fibers. The nano materials generated using this technology have a large surface area and are highly porous making it very useful in many applications in diverse fields such as energy storage, healthcare, biotechnology, environmental engineering, defense and security. The production of the fibers and the morphology can be easily controlled by modifications to the processing parameters. The relatively high production rate and simplicity of the setup makes electrospinning highly attractive. This review summarizes the effect of various processing parameters on the effective generation of nanofibers. By simple modifications to the electric field inside the electrospinning chamber the fiber collection can be easily controlled. In addition, the various applications of electrospun fibers in electronic devices, environmental sensors and filters, energy storage, and in biomedicine such as in tissue engineering, drug delivery and enzyme encapsulation are examined and the current research in each field is also explored in this review.
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Department of Physics and Technology, University of Bergen, Bergen, Norway.
The yield of charged particles associated with high-p_{t} trigger particles (8<p_{t}<15 GeV/c) is measured with the ALICE detector in Pb-Pb collisions at sqrt[s_{NN}]=2.76 TeV relative to proton-proton collisions at the same energy. The conditional per-trigger yields are extracted from the narrow jetlike correlation peaks in azimuthal dihadron correlations. In the 5% most central collisions, we observe that the yield of associated charged particles with transverse momenta p_{t}>3 GeV/c on the away side drops to about 60% of that observed in pp collisions, while on the near side a moderate enhancement of 20%-30% is found.
B Abelev,
A Abrahantes Quintana,
D Adamová,
A M Adare,
M M Aggarwal,
G Aglieri Rinella,
A G Agocs,
A Agostinelli,
S Aguilar Salazar,
Z Ahammed,
N Ahmad,
A Ahmad Masoodi,
S U Ahn,
A Akindinov,
D Aleksandrov,
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E Almaráz Aviña,
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V Altini,
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C Anson,
T Antičić,
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F Bellini,
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S Beole,
I Berceanu,
A Bercuci,
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B Boyer,
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X Zhang,
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D Zhou,
Y Zhou,
X Zhu,
A Zichichi,
A Zimmermann,
G Zinovjev,
Y Zoccarato,
M Zynovyev
Lawrence Livermore National Laboratory, Livermore, California, USA.
The ALICE Collaboration has studied J/ψ production in pp collisions at sqrt[s]=7 TeV at the LHC through its muon pair decay. The polar and azimuthal angle distributions of the decay muons were measured, and results on the J/ψ polarization parameters λ_{θ} and λ_{ϕ} were obtained. The study was performed in the kinematic region 2.5<y<4, 2<p_{t}<8 GeV/c, in the helicity and Collins-Soper reference frames. In both frames, the polarization parameters are compatible with zero, within uncertainties.
Animal Biotechnology Laboratory, Eastern Regional Station, National Dairy Research Institute, Kalyani, West Bengal, India;
The purpose of this study was to determine the effect of heparin-binding oviducal secretory proteins as a media supplement on IVF and embryo development in cattle. The native proteins were isolated from oviducts collected from a slaughterhouse by repeated freeze-thawing and precipitated by ammonium sulfate (60%) followed by dialysis overnight in 10mM phosphate buffer solution containing 1mM PMSF at pH 7.0 at 4°C. The dialyzed product was then passed through a high-performance liquid chromatography system with a HiTrap™ heparin prepacked column (GE Healthcare, Piscataway, NJ) equilibrated with 10mM phosphate buffer containing 1mM EDTA. The collected protein fractions [heparin-binding protein (HBP), heparin-unbinding protein (HUBP)] and total protein (TP) were run on SDS-PAGE. The SDS-PAGE profile of the eluted HBP fraction contained 5 major protein bands visible between approximately 66 to 97kDa, the profile of the HUBP fraction contained 2 bands nearer to approximately 66kDa and in TP, all 7 bands were visible between approximately 60 to 95kDa. All 3 fractions (i.e. HBP, HUBP and TP) were used in 3 different concentrations (0, 1, 10 and 30μgmL(-1)) for in vitro maturation, sperm preparation, IVF and in vitro culture of cleaved embryos. Cumulus-oocyte complexes were collected from slaughterhouse ovaries, washed 4 to 5 times and cultured in maturation media for 24h in a 5% CO(2) incubator at 38.5°C with maximum humidity. In vitro-matured oocytes were co-incubated with capacitated sperm in Fert-BO media at 38.5°C in 5% CO(2) in air with maximum humidity. After 18h, oocytes were washed thoroughly and cultured in embryo development media for cleavage. After 40 to 42h, cleavage was observed and embryos were transferred into the replacement media for further development. In the HBP group, overall cleavage rates (%) were 63±3.0, 53±2.6, 65±4.7 and 43±0.6 and blastocyst formation (%) was 17±1.6, 23±2.5, 25±2.2 and 24±1.7 in 0, 1, 10 and 30μgmL(-1) concentrations, respectively. However, in the HUBP group, the overall cleavage rates (%) were 66±4.3, 56±2.0, 60±2.7 and 32±0.8 and blastocyst formation (%) was 18±1.1, 25±1.8, 24±2.2 and 14±0.8 in 0, 1, 10 and 30μgmL(-1) concentrations, respectively. In the TP group, cleavage rates (%) were 67±4.9, 60±4.1, 73±3.2 and 41±1.6 and blastocyst formation (%) was 19±1.1, 18±1.9, 27±2.2 and 20±1.6 in 0, 1, 10 and 30μgmL(-1) concentrations, respectively. These results indicate that 10μgmL(-1) of the total oviductal secretory protein fraction used as a media supplement significantly increased (P<0.01) the cleavage rate and blastocyst formation as compared with the HBP and HUBP fractions.
Eastern Regional Station, National Dairy Research Institute, Kalyani, West Bengal, India;
The objective of this study was to determine the effect of oviducal secretory proteins (cOSP) as a media supplement on in vitro embryo development in cattle. Oviducal secretory proteins were collected from slaughterhouse oviducts by repeated freeze-thaw process and purified by ammonium sulfate precipitation (30%, 40%, 50% and 60%) followed by dialysis in 50mM tris-HCl (pH 7.0) buffer. Dialyzed products were further purified by SP Sephadex cation exchange column and diethylaminoethyl cellulose (DEAE) anion exchange column, extensively washed and eluted by 50mM tris-HCl (pH 7.0) containing 1.5M NaCl. Both bound and unbound proteins were collected separately, dialyzed in phosphate buffer saline and quantified. Presence of protein was confirmed by running sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and OSP bands between ~66kD to ~97kD were found. The pooled purified cOSP were used as a media supplement in 3 different concentrations (0, 10, 50 and 100μg mL(-1)) for in vitro production (n=3) of cattle embryos. Cumulus-oocyte complexes (n=370) were collected from slaughterhouse ovaries, washed thoroughly and cultured in maturation media for 24h in 5% CO(2) at 38.5°C with maximum humidity. In vitro-matured oocytes were fertilized with in vitro capacitated sperm in Fert-BO media at 38.5°C in 5% CO(2). After 16 to 18h, oocytes were washed and cultured in embryo development media for cleavage. After 40 to 42h, cleavage was observed and embryos were transferred into the replacement media for further development. A total of 68.42%, 69.31%, 61.82% and 41.67% cleavage rate and 15.38%, 21.31%, 14.70% and 15.0% blastocyst rate was observed at concentrations of 0, 10, 50 and 100μg mL(-1), respectively. These results indicate that addition of cOSP at 10μg mL(-1) increased blastocyst formation significantly (P<0.05) compared with 0, 50 and 100μg mL(-1) and increased cleavage rate significantly (P<0.05) compared with 50 and 100μg mL(-1).
A K Dang,
S Prasad,
K De,
S Pal,
J Mukherjee,
I V R Sandeep,
G Mutoni,
M M Pathan,
M Jamwal,
S Kapila,
R Kapila,
H Kaur,
S Dixit,
A K Mohanty,
B S Prakash
National Dairy Research Institute, Karnal, Haryana, India.
To study the effect of vitamin E (VE), copper (Cu) and zinc (Zn) supplementation on the in vitro phagocytic activity (PA) and lymphocyte proliferation response (LPR) of blood neutrophils and lymphocytes, thirty Sahiwal pregnant cows (six in each group) in their late gestation at 30 days before the expected date of calving were selected from the NDRI experimental herd and supplemented with various micronutrients from 30 days before calving to 45 days after calving. Cows were supplemented individually with VE (1000 IU/cow/day), Cu (20 ppm/cow/day) and Zn (80 ppm/cow/day) and also with a combination of VE, Cu and Zn to study cumulative effect of all micronutrients. One group without any supplementation acted as a control. Blood neutrophils and lymphocytes were isolated and studied for their PA and LPR. Supplementation of micronutrients like VE, Cu, Zn and a combination of all these nutrients significantly (p < 0.01) increased the PA of experimental cows as compared to control (unsupplemented) cows during the pre-partum period. During post-partum, all the micronutrients (VE, Cu, Zn and their combination) showed a significant (p < 0.01) increase in the PA of experimental cows as compared to control cows. Of all the groups, significant (p < 0.01) and maximum PA was observed in the combination group followed by Zn-supplemented group during both the pre- and post-partum period. A significant (p < 0.01) increase in LPR of B lymphocytes was observed in combination-supplemented group during the pre-partum period and during both the pre- and post-partum period in the Cu-supplemented group.
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