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| 1 | 0 | 0 | 1045 | 1 | 15439 | [Update] | |
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Latest Paper:
Br J Radiol. 2012 May 9;:
22573294
Department of Nuclear Medicine, Furness General Hospital, Barrow-in-Furness, UK.
ROCView has been developed as an image display and response capture (IDRC) solution to image display and consistent recording of reader responses in relation to the free-response receiver operating characteristic paradigm. A web-based solution to IDRC for observer response studies allows observations to be completed from any location, assuming that display performance and viewing conditions are consistent with the study being completed. The simplistic functionality of the software allows observations to be completed without supervision. ROCView can display images from multiple modalities, in a randomised order if required. Following registration, observers are prompted to begin their image evaluation. All data are recorded via mouse clicks, one to localise (mark) and one to score confidence (rate) using either an ordinal or continuous rating scale. Up to nine "mark-rating" pairs can be made per image. Unmarked images are given a default score of zero. Upon completion of the study, both true-positive and false-positive reports can be downloaded and adapted for analysis. ROCView has the potential to be a useful tool in the assessment of modality performance difference for a range of imaging methods.
S Chatrchyan,
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W H Smith,
J Swanson
Yerevan Physics Institute, Yerevan, Armenia.
A search for a Higgs boson in the four-lepton decay channel H→ZZ, with each Z boson decaying to an electron or muon pair, is reported. The search covers Higgs boson mass hypotheses in the range of 110<m_{H}<600 GeV. The analysis uses data corresponding to an integrated luminosity of 4.7 fb^{-1} recorded by the CMS detector in pp collisions at sqrt[s]=7 TeV from the LHC. Seventy-two events are observed with four-lepton invariant mass m_{4ℓ}>100 GeV (with 13 below 160 GeV), while 67.1±6.0 (9.5±1.3) events are expected from background. The four-lepton mass distribution is consistent with the expectation of standard model background production of ZZ pairs. Upper limits at 95% confidence level exclude the standard model Higgs boson in the ranges of 134-158 GeV, 180-305 GeV, and 340-465 GeV. Small excesses of events are observed around masses of 119, 126, and 320 GeV, making the observed limits weaker than expected in the absence of a signal.
S Chatrchyan,
V Khachatryan,
A M Sirunyan,
A Tumasyan,
W Adam,
T Bergauer,
M Dragicevic,
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W H Smith,
J Swanson,
M Weinberg
Yerevan Physics Institute, Yerevan, Armenia.
A search for signatures of extra spatial dimensions in the diphoton invariant-mass spectrum has been performed with the CMS detector at the LHC. No excess of events above the standard model expectation is observed using a data sample collected in proton-proton collisions at sqrt[s]=7 TeV corresponding to an integrated luminosity of 2.2 fb^{-1}. In the context of the large-extra-dimensions model, lower limits are set on the effective Planck scale in the range of 2.3-3.8 TeV at the 95% confidence level. These limits are the most restrictive bounds on virtual-graviton exchange to date. The most restrictive lower limits to date are also set on the mass of the first graviton excitation in the Randall-Sundrum model in the range of 0.86-1.84 TeV, for values of the associated coupling parameter between 0.01 and 0.10.
Ups J Med Sci. 2012 Apr 17;:
22509804
Inga Nazarenko,
Sanna-Maria Hede,
Xiaobing He,
Anna Hedrén,
James Thompson,
Mikael S Lindström,
Monica Nistér
Department of Oncology-Pathology, Karolinska Institutet, CCK R8:04, Karolinska University Hospital Solna , SE-17176 Stockholm , Sweden.
Abstract The family of platelet-derived growth factors (PDGFs) plays a number of critical roles in normal embryonic development, cellular differentiation, and response to tissue damage. Not surprisingly, as it is a multi-faceted regulatory system, numerous pathological conditions are associated with aberrant activity of the PDGFs and their receptors. As we and others have shown, human gliomas, especially glioblastoma, express all PDGF ligands and both the two cell surface receptors, PDGFR-α and -β. The cellular distribution of these proteins in tumors indicates that glial tumor cells are stimulated via PDGF/PDGFR-α autocrine and paracrine loops, while tumor vessels are stimulated via the PDGFR-β. Here we summarize the initial discoveries on the role of PDGF and PDGF receptors in gliomas and provide a brief overview of what is known in this field.
Health and Social Care Institute, School of Health and Social Care, Teesside University, Middlesbrough, TS1 3BA, UK.
Iowa State University, Department of Natural Resource Ecology and Management, 339 Science Hall II, Ames, IA 50010, USA.
Residents, developers and civic officials are often faced with difficult decisions about appropriate land uses in and around metropolitan boundaries. Urban expansion brings with it the potential for negative environmental impacts, but there are alternatives, such as conservation subdivision design (CSD) or low-impact development (LID), which offer the possibility of mitigating some of these effects at the development site. Many urban planning jurisdictions across the Midwest do not currently have any examples of these designs and lack information to identify public support or barriers to use of these methods. This is a case study examining consumer value for conservation and low-impact design features in one housing market by using four different valuation techniques to estimate residents' willingness to pay for CSD and LID features in residential subdivisions. A contingent valuation survey of 1804 residents in Ames, IA assessed familiarity with and perceptions of subdivision development and used an ordered value approach to estimate willingness to pay for CSD and LID features. A majority of residents were not familiar with CSD or LID practices. Residents indicated a willingness to pay for most CSD and LID features with the exception of clustered housing. Gender, age, income, familiarity with LID practices, perceptions of attractiveness of features and the perceived effect of CSD and LID features on ease of future home sales were important factors influencing residents' willingness to pay. A hypothetical referendum measured willingness to pay for tax-funded conservation land purchases and estimated that a property tax of around $50 would be the maximum increase that would pass. Twenty-seven survey respondents participated in a subsequent series of experimental real estate negotiations that used an experimental auction mechanism to estimate willingness to pay for CSD and LID features. Participants indicated that clustered housing (with interspersed preserved forest or open space areas), rain gardens, and neighborhood streams with a forested buffer were the features they were most willing to pay for. Participants were not willing to pay for neighborhood streams without buffers. Finally, a spatial hedonic price model using 2093 homes in Ames, IA was used to estimate the effect of public and private open space on housing values. The model indicated that presence of neighborhood association-owned forest and water features as well as proximity to public parks had significant positive effects on housing prices. However, proximity to a public lake had a negative effect on home values. The four methods used in this study include both stated and revealed preference techniques. Although the relative magnitude of value expressed varied, all methods indicated that residents value CSD and LID subdivision features. Subdivision features that included explicit environmental benefits were also consistently preferred over features that did not. Familiarity with alternative designs was an important factor influencing resident willingness to pay for neighborhood features, and developers and civic officials should consider ways to educate citizens about CSD and LID development techniques to increase interest in these designs.
Environ Sci Technol. 2012 Apr 9;:
22486760
Sarit Leat Kaserzon,
Karen Kennedy,
Darryl W Hawker,
Jack Thompson,
Steve Carter,
Anthony C Roach,
Kees Booij,
Jochen F Mueller
Perfluorinated chemicals (PFCs) are emerging environmental contaminants with a global distribution. Due to their moderate water solubility, the majority of the environmental burden is assumed to be in the water phase. This work describes the application of the first passive sampler for the quantitative assessment of concentrations of perfluorinated alkylcarboxylates (PFCAs) and sulfonates (PFSAs) in water. The sampler is based on a modified Polar Organic Chemical Integrative Sampler (POCIS) with a weak anion exchange sorbent as a receiving phase. Sampling rates were between 0.16 and 0.37 L d 1, and the duration of the kinetic sampling stage was between 2.2 and 13 d. A field deployment in the most urbanised estuary in Australia (Sydney Harbour) showed trace level concentrations from passive samplers (0.1 - 12 ng L-1), in good agreement with parallel grab sampling (0.2 - 16 ng L-1). A separate field comparison of the modified POCIS with standard POCIS suggests the latter may have application for PFC sampling, but with a more limited range of analytes than the modified POCIS which contains a sorbent with a mixed mode of action.
J Physiol. 2012 Mar 19;:
22431336
Eric J Stöhr,
Barry McDonnell,
Jane Thompson,
Keeron Stone,
Tom Bull,
Rory Houston,
John Cockcroft,
Rob Shave
Cardiff Metropolitan University;
Individuals with high aerobic fitness have lower systolic left ventricular strain, rotation and twist ('LV mechanics') at rest, suggesting a beneficial reduction in LV myofibre stress and more efficient systolic function. However, the mechanisms responsible for this functional adaptation are not known and the influence of aerobic fitness on LV mechanics during dynamic exercise has never been studied. We assessed LV mechanics, LV wall thickness and dimensions, central augmentation index (AIx), aortic pulse wave velocity (aPWV), blood pressure and heart rate in 28 males (age: 21±2 yrs) with a consistent physical activity level (no change >6months). Individuals were examined at rest and during exercise (40% peak exercise capacity) and separated post hoc into a moderate and high aerobic fitness group (VO2peak: 49±5 and 63±7 ml/kg/min, respectively, p<0.0001). At rest and during exercise, there were no significant differences in gross LV structure, AIx, blood pressure or heart rate (p>0.05). However, for the same AIx, the high VO2peak group had significantly lower LV apical rotation (p=0.002) and LV twist (p=0.003) while basal rotation and strain indices did not differ between groups (p>0.05). We conclude that young males with high aerobic fitness have lower LV apical rotation at rest and during sub-maximal exercise that can occur without changes in gross LV structure, arterial haemodynamics or heart rate. The findings suggest a previously unknown type of physiological adaptation of the LV that may have important implications for exercise training in older individuals and patient populations in which exercise training has previously failed to show clear benefits for LV function.
Robinson Institute, The University of Adelaide, Adelaide, South Australia, Australia.
Current embryo culture media are based on the carbohydrate metabolism of embryos. However, little is known about the metabolism of endogenous lipids. This is surprising given the high intracellular lipid densities of embryos of some species and the potential for ATP production via β-oxidation. L-carnitine is a β-oxidation co-factor that is absent in most culture media. The aim of this study is to investigate the influence of carnitine supplementation±carbohydrates on bovine embryo development. Abattoir-derived cattle cumulus-oocyte complexes were cultured and fertilised (Sutton-McDowall et al. 2006 Biol. Reprod. 74, 881-888). Post-fertilisation (24h), presumptive zygotes were transferred into an amino acid-free cleavage media±carbohydrates (glucose, lactate and pyruvate) ±5mM carnitine and cultured for 4 days. The absence of carbohydrates during culture resulted in embryos arresting at the 2- and 4-cell stages. Remarkably,+carnitine significantly increased development to the morula stage compared with +carbohydrates alone (20.4±3% vs 4.7±2.5% morula development; P<0.001). The combination of carbohydrates and carnitine supplementation further improved embryo development, with 14-fold more embryos reaching the morula stage after culture in the +carbohydrates +carnitine group compared with the +carbohydrates group (+carbohydrates=3.1±1.9 vs +carbohydrates +carnitine=43.8±9.1% morula development; P<0.05). The beneficial effects of carnitine supplementation on embryo development were reversed when embryos were cultured in presence of etomoxir, a non-reversible inhibitor of the rate-limiting enzyme of β-oxidation (development to 8-cell stage;+carnitine=33.9±8% vs +carnitine +etomoxir=19.2±4.9%; P<0.05). Intracellular lipid content of embryos +carnitine was determined by culturing presumptive zygotes in media -carbohydrates±carnitine for 24h. Lipid content of embryos was determined by measuring BODIPY 493/503 dye fluorescence. Carnitine supplementation reduced fluorescence intensity 1.8-fold (P<0.001). Adenosine triphosphate and ATP:ADP levels were measured in embryos after 24h of culture±carbohydrates±carnitine. While there was a trend for +carnitine to increase ATP levels (P=0.09), ADP levels were higher and ATP:ADP ratio were 1.9-fold lower (main effect, P<0.05) compared with embryos cultured in -carnitine. This indicates +carnitine embryos were more metabolically active, with higher rates of ATP-ADP conversion. We have shown carnitine supplementation supports pre-compaction embryo development and there is an additive effect of +carnitine +carbohydrate on early embryo development. This is most likely through increased β-oxidation levels within embryos. Current disparities between in vivo and in vitro embryo production, in particular increased lipid content (Romek et al. 2010 Theriogenology 74, 265-276) and decreased developmental potential of in vitro-produced embryos, may be an artefact resulting from limited lipid oxidation in vitro.
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