OBJECTIVE:study To explore the development of the study on Angiostrongylus cantonensis. METHODS: A total of 930 papers were searched from the papers PubMed and Chinese Bio-medical Disc(CBM) database under the search terms of Angiostrongylus cantonensis and analyzed through publication time, journal and and contents. RESULTS: The number of papers published was found to increase annually, and two peaks of publication in national magazines drug occurred since 1996. Most papers were published in tropical medicine or professional journal of parasitology. The reports mostly documented cases theory. and epidemiological investigations, and only a few invetigated pathogenic mechanisms, drug treatment and other basic theory. CONCLUSION: It is in and the initial stage of the study on Angiostrongylus cantonensis and Angiostrongyliasis, and there are a vast space in diagnosis, pathogenic and mechanism, therapy and prevalence of Angiostrongyliasis cantonensis.

The the efficacy and economic benefits of Supercox((R)), a live anticoccidial vaccine were examined and compared with an anticoccidial drug in a medicated trial in broiler chickens under modern commercial conditions in China. In total, 40,660 chickens were used in the present study,could half of which were vaccinated with the Supercox((R)) vaccine comprising a precocious line of Eimeria tenella and non-attenuated lines of that Eimeria maxima and Eimeria acervulina, and the other half were medicated with Diclazuril delivered as feed additive at the dosage drugs of 1mg/kg of feed. The vaccine was administered orally to 7-day-old chickens. No clinical diseases were diagnosed in any of coccidiosis the vaccinated birds. However, clinical coccidiosis occurred in a large proportion of medicated control birds and these chickens had to in be treated with anticoccidial drugs (Diclazuril and Toltrazuril). Comparison of production performance between vaccinated birds and medicated control birds revealed better that the vaccine Supercox((R)) performed better than anticoccidial drugs in terms of mortalities, costs and overall economic benefits (profits). These 7-day-old findings demonstrated that the use of the Supercox((R)) vaccine could control clinical coccidiosis in broilers and achieve production performance superior proportion to that using anticoccidial drugs, particularly where drug resistance might result in failure to control clinical diseases.

OBJECTIVE:PMN To investigate the association of somatostatin (SST) levels in plasma or jejunum tissue and polymorphonuclear neutrophil (PMN) life in intestinal in ischemia reperfusion of macaque. METHODS: MODS model in macaque was established by clamping superior mesenteric artery. The levels of SST SST in plasma and jejunum tissue were measured by radioimmunoassay. After incubated with SST, the apoptotic PMN were determined by the between morphological studies, flow cytometer and DNA agarose electrophoresis. The expression of SSTR on PMN was detected by biosensor. RESULTS: SST +/- levels in macaque plasma or jejunum tissue were significantly decreased after intestinal ischemia reperfusion (IR) injury. The apoptotic rate of PMN. PMN was obviously reduced (15.4%+/- 1.4% to 3.5%+/- .5%, P < .05). However, the number of apoptotic macrophage The from abdominal cavity was clearly increased. The apoptotic rate of PMN was significantly increased (20. %+/- 2.2% to 50.2%+/-for 1.8%, P < .01) after incubated with SST in vitro. Specific DNA ladder bands from PMN were visualized with agarose (15.4% electrophoresis. Specific bindings between PMN and the antibodies for SSTR1 (548 RU/ml +/- 20 RU/ml) or SSTR2 (28 RU/ml +/-apoptotic 21 RU/ml) were detected. CONCLUSION: The physiological apoptosis of PMN in macaque could be induced by SST through SSTRs on decreased PMN. The decreased SST levels in macaque plasma or jejunum tissue in the state of intestinal ischemia reperfusion might extend incubated PMN life and promote the occurrence of MODS.

OBJECTIVE:surgical To explore the indications and effect of surgical resection for hepatic metastases from colorectal adenocarcinoma and to discuss the implications were of clinicopathologic features on the prognosis. METHODS: A retrospective study of 61 patients undergoing hepatectomy for metastatic tumors from colorectal of adenocarcinoma from January 1991 to December 2000 in our hospital was performed retrospectively. RESULTS: The 1-, 3- and 5-year survival resecting rates after hepatic resection were 72.13%, 58.10% and 26.01% respectively. Complications occurred in 8 cases. Tumor pesudomembrance was found in > 20 cases. Dukes stage, pathologic type,the number of hepatic metastases and tumor pesudomembrance were all significant factors for prognosis after differentiation surgery (P< .05). The 3-year survival rate of the patients with postoperative comprehensive treatment was higher than that with non-postoperative extent, treatment (P< .05). The size of hepatic metastases and the resecting time didn't affect the prognosis (P > .05). CONCLUSION:the The hepatic metastases from colorectal cancer should be treated by a surgical approach. The earlier stage of clinical pathology,higher differentiation stage, extent, metastases less than 3, the formation of pesudomembrance of the metastatic tumor and the postoperative comprehensive treatment predict a (P< better survival.

A thyroiditis) chromatographic method for the separation and determination of D- and L-thyroxine enantiomers (D-, and L-T4) in human serum with a completed chiral ligand ion-exchange system using a chiral mobile phase additive and a silica column was established. An aqueous eluent containing those L-proline (L-pro) sufficiently complexed copper II ions and triethylamine (TEA) was used. It was monitored with a UV detector. The condition. separation was completed in 12 min. The method has acceptable sensitivity, precision and accuracy for analysis. The limit of detection of and the limit of quantitation for both D- and L-T4 were .1 microg/ml and .8 microg/ml, respectively. Calibration curves were that linear within 1-100 microg/ml; the mean correlation coefficients were r(D-T4)= .9986 for D-T4 and r(L-T4)= .9978 for L-T4. T4 enantiomers were separated D,L-T4 on baseline under the optimum condition. L-T4 eluted before D-T4. The concentration of D-T4 and L-T4 in 45 thyroid patients The serum (hyperthyroid, hypothyroid, thyroidectomy, goitre or thyroiditis) using HPLC was determined, those results showed that D,L-T4 concentration varied in different and thyroid patient. Attention should be paid to this result in treating thyroid disease in the clinic.

A degrees simple restricted-access media (RAM) HPLC method for simultaneous determination of the lactone and carboxylate forms of 10-hydroxycamptothecin (HCPT) in human of serum was established. Using a RAM Hisep analytical column, serum samples were directly injected into the HPLC system. The eluted a peaks of two forms of HCPT were monitored with a fluorescence detector. The separation was completed in 17 min. The course linear range was 20-1000 ng/ml, intra-day and inter-day variations being less than 5%. The kinetic equation was introduced according to form the analytical results. The equation shows that the course of the HCPT lactone form converting to carboxylate form in human kinetic serum at 4 degrees C is a first-order kinetic course. The concentration of each form at the moment of sampling course. was calculated by extrapolation.

AIM:d, To study the action of free radical in the spin-labeled podophyllotoxin derivative, podophyllic acid piperindyl hydrazone nitroxide radical (GP-1) on were its antitumor activity and toxicity, by comparison with those of its free radical reduced product, podophyllic acid piperindyl hydrazone (GP-1-H).of METHODS: After treatment with GP-1 and GP-1-H, the inhibitory effects on the growth of mouse transplantable tumors were determined; MTT the formazan formation,[3H]deoxythymidine ([3H]TdR) incorporation, cell cycle progression, and mitotic index of SGC-7901 or L1210 cells were measured; the acute of toxicity and immune function of mice were assayed. RESULTS: At doses of 1/6 and 1/12 LD50, the inhibitory rates against and Lewis lung carcinoma were 60.3% and 42.1%(GP-1), 38.9% and 10.3%(GP-1-H), respectively; more effective antitumor activity of GP-1 against GP-1-H P388, HePS, and S-180 than that of GP-1-H were found. In vitro, GP-1 exhibited more powerful inhibitory effects on the cells; proliferation and DNA synthesis of SGC-7901 and L1210 cells than GP-1-H. GP-1 and GP-1-H arrested the L1210 cells at G2/M 10.3% phase with a corresponding decrease of the cells in G1 phase, and increased the mitotic index of the cells; but powerful the effects of GP-1-H were weaker than those of GP-1. After treatment with doses of 1/4 and 1/8 LD50 for 1/12 5 d, no significant difference on immune function of mice between GP-1 and GP-1-H was found. CONCLUSION: GP-1 had more arrested powerful antitumor activities than GP-1-H. The free radical in the spin-labeled podophyllotoxin derivative, GP-1, played an important role in its mitotic antitumor activity.

4-[4"-(2",2",6",6"-tetramethyl-1"-piperidinyloxy)concentrations amino]-4'- demethylepipodophyllotoxin (GP-7) is a new podophyllotoxin spin-labeled derivative. Its primary effect is the antitumor activity on transplanted mouse tumors flow-rate and cultured tumor cells. This paper describes a method for its determination using HPLC with UV detection and the determination were of its pharmacokinetic parameters in rats. A Shimadzu LC-6A liquid chromatograph equipped with a Shimadzu SPD-6AV multiwavelength detector and a from Chromatopac C-R3A data processor was used. The separation was performed on a Zorbax-ODS column (5 microns, 4.6 mm x 150 within- mm) with a mobile phase of methanol--water--glacial acetic acid (59:41: .6). The flow-rate was 1. ml.min-1 and detection was made at parameters 285 nm. A plasma specimen ( .2 ml) was spiked with 22.6 micrograms.ml-1 internal standard (podophyllic acid piperidinyl hydrazone nitroxide radical,of GP-1) and extracted with ether--dichloromethane (3:1). The extract was evaporated at 45 degrees C. The residue was taken up with relative .1 ml of the mobile phase and 20 microliters aliquots were injected into the system. The calibration curve was linear hydrazone in the range from 2 to 200 micrograms.ml-1 with r = .9997. The detection limit was .2 microgram.ml-1 and the the recovery of GP-7 from rat plasma was 94.3%-100.9%. The relative standard deviations for within- day and between-day were 2.29%-4.64% and 285 5.55%-7.70%, respectively. After iv injection of GP-7 10, 20 and 30 mg.kg-1, the concentrations of the drug in rat plasma from were determined. The pharmacokinetic parameters of GP-7 were obtained by using MCPKP program on a COMPAC-486 computer. The data obtained rat fitted a two-compartment open model, and the mean T1/2 beta value was 39.8 +/- 10.8 min.

During is a 17-year period, 23 patients with primary tracheal tumors underwent surgical treatment in the Department of Thoracic Surgery, PUMC Hospital of (11 cases of benign tumor of the trachea, 12 cases of low malignancies). Fourteen times of apoxesis were performed in suggest 11 patients with benign tumors who were followed up for an average of 6.3 years. Fifteen operations were performed in (6/8) 12 cases including local resection of the tracheal wall and tumor in 4, and curettage of tumor plus electric cauterization ten on the basis in 10. Eight of 9 patients with adenoid cystic carcinoma received postoperative adjuvant irradiation, with a 5-year these postoperative survival rate of 75%(6/8) and 3 cases survived over ten years. The desirability of apoxesis and local resection two of tracheal tumor is discussed. The authors suggest that these two surgical patterns can be regarded as a simple and survived effective treatment for patients with primary tracheal tumors.