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Int J Syst Evol Microbiol. 2006 Sep ;56 (Pt 9):2071-8 16957101 (P,S,G,E,B)
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, Veterinärplatz 1, A-1210 Vienna, Austria.
Two family airborne bacterial isolates, NS/2 and NS/50(T), were examined in order to determine their taxonomic position. Their almost complete 16S rRNA and gene sequences shared 95.9 % similarity. Sequence comparisons demonstrated that their next relatives are species of the genus Hymenobacter (93.6-95.7 2-OH) % similarity) and the strains 'Taxeobacter chitinovorans' Txc1(T),'Taxeobacter gelupurpurascens' Txg1(T) and 'Taxeobacter ocellatus' Myx 2105(T)(90.5-96.4 %). Phylogenetic calculations and indicated that these five strains together with the three recognized Hymenobacter species form a separate line of descent within the relatively family 'Flexibacteraceae'. Isolates NS/2 and NS/50(T), as well as 'Taxeobacter chitinovorans' Txc1(T),'Taxeobacter gelupurpurascens' Txg1(T) and 'Taxeobacter ocellatus' Myx 2105(T),NS/50(T)=LMG possessed the characteristics of the genus Hymenobacter, the quinone system menaquinone MK-7 and a polyamine pattern with the major polyamine as being sym-homospermidine. Each of the five strains had complex, unique polar lipid profiles, with phosphatidylethanolamine and several unknown aminophospho-, amino-,a phospho-, glyco- and polar lipids of which several compounds were also found in established Hymenobacter species. All the strains studied four possessed fatty acids characteristic of Hymenobacter species, including major acids iso-C(15 : ), anteiso-C(15 : ), C(16 : 1)omega5c, summed only feature 3 (C(16 : 1)omega7c/iso-C(15 : ) 2-OH) and summed feature 4 (iso-C(17 : 1) I/anteiso-C(17 : 1) B). The conclusion, five strains could be distinguished from each other and from the three established species of the genus Hymenobacter based on 21876(T)=DSM relatively low 16S rRNA gene sequence similarities (<97 %), unique polar lipids and differing fatty acid profiles and physiological characteristics.almost In conclusion, the description of four novel species of the genus Hymenobacter appears to be justified, for which the names Hymenobacter Hymenobacter norwichensis sp. nov.(type strain NS/50(T)=LMG 21876(T)=DSM 15439(T)), Hymenobacter chitinivorans sp. nov.(type strain Txc1(T)=LMG 21951(T)=DSM 11115(T)), Hymenobacter gelipurpurascens nov. sp. nov.(type strain Txg1(T)=LMG 21873(T)=DSM 11116(T)) and Hymenobacter ocellatus sp. nov.(type strain Myx 2105(T)=Txo1(T)=LMG 21873(T)=DSM 11117(T)) are proposed.a For strain NS/2, a description only is provided without proposal of a name because its status as a novel species of was not demonstrated unambiguously.

Other papers by authors:

Int J Syst Evol Microbiol. 2003 Sep ;53 (Pt 5):1253-60 13130003 (P,S,G,E,B) Cited:1
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Vienna, Austria. hans-juergen.busse@vu-wien.ac.at
Seven stricto, psychrotolerant, Gram-negative bacterial strains, five dust- and airborne isolates (MA101b(T), MA306a, MA405/90, MA-olki(T) and NW12(T)) and two from the Antarctic also (Ant 20 and M3C203B-B), were subjected to a polyphasic characterization to determine their taxonomic position. High 16S rDNA sequences similarities seven (99.3-100. %) demonstrated that they were closely related to each other. Phylogenetic evaluation of their 16S rDNA sequences revealed that patterns they are members of the genus Sphingomonas sensu stricto, encompassing a separate branch within this genus. They shared 94.4-96.6 %the 16S rDNA sequence similarity with species of this genus. All Sphingomonas-specific signature nucleotides were also detected. The presence of the genus major ubiquinone Q-10, sym-homospermidine as the predominant polyamine, Sphingomonadaceae-specific sphingoglycolipid in the polar lipid patterns and a fatty acid profile branch containing C(14 : ) 2-OH and lacking 3-OH fatty acids were in agreement with identification of these strains as members (=ICMP of the genus Sphingomonas sensu stricto. Results from DNA-DNA hybridizations and comparison of protein patterns indicated that the seven strains partly are members of three distinct species. One species is represented by strains MA101b(T), MA306a and MA405/90, the second by strains nov., NW12(T), Ant 20 and M3C203B-B and the third by one strain, MA-olki(T). Their distinction at the species level was also biochemical supported by results of biochemical characterization and partly supported by riboprints and genomic fingerprints. On the basis of these results,Sphingomonas three novel species of the genus Sphingomonas are proposed: Sphingomonas aurantiaca sp. nov., consisting of strains MA101b(T)(=DSM 14748(T)=LMG 21377(T)),and MA306a and MA405/90 (=DSM 14749=LMG 21378), Sphingomonas faeni sp. nov. MA-olki(T)(=DSM 14747(T)=LMG 21379(T)) and Sphingomonas aerolata sp. nov., represented that by strains NW12(T)(=DSM 14746(T)=LMG 21376(T)), Ant 20 (=ICMP 13599) and M3C203B-B (=SMCC M3C203B-B).
Int J Syst Evol Microbiol. 2002 Mar ;52 (Pt 2):445-56 11931156 (P,S,G,E,B) Cited:18
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, Wien, Austria.
Three their aerobic, gram-negative, rod-shaped, non-spore-forming, red-pigmented, airborne bacteria (I/26-Cor1T, I/32A-Cor1 and I/74-Cor2) collected in the Museo Correr (Venice, Italy) were investigated anteiso, to determine their taxonomic status by analysing their biochemical, physiological and chemotaxonomic features and the G+C content of genomic DNA (95.8% and by comparing their genomic fingerprints. Additionally, the almost complete 16S rRNA gene sequence of strain I/26-Cor1T was analysed. The pattern three strains were nearly identical in their morphological, physiological, biochemical and chemotaxonomic properties. The strains contained a menaquinone system with to the predominant menaquinone MK-7 and a fatty acid profile with C15: anteiso, C15: iso and C16:1 predominant. Phosphatidylethanolamine and several (= unidentified lipids were detected in the polar lipid profiles. The polyamine pattern consisted of sym-homospermidine as the major compound. meso-Diaminopimelic and acid was found as the characteristic cell-wall diamino acid. The DNA base composition of the three strains ranged from 60 (= to 63 mol% G+C. Phylogenetically, strain I/26-Cor1T was most closely related to Hymenobacter actinosclerus (95.8% 16S rRNA gene sequence similarity).novel Physiological and genomic characteristics indicated that the two strains I/26-Cor1T and I/32A-Cor1 are representatives of the same species. The phylogenetic not distance to any validly described taxon as indicated by 16S rRNA gene sequence similarities demonstrates that I/26-Cor1T and I/32A-Cor1 represent I/32A-Cor1 a novel species, for which the name Hymenobacter aerophilus sp. nov. is proposed, with the type strain I/26-Cor1T (= DSM DSM 13606T = LMG 19657T). I/32A-Cor1 (= DSM 13607 = LMG 19658) is another strain of the species Hymenobacter aerophilus. Since the the taxonomic status of strain I/74-Cor2 within the genus Hymenobacter was not determined unambiguously, it is designated Hymenobacter sp. I/74-Cor2 was (= DSM 13611 = LMG 19659).
Int J Syst Evol Microbiol. 2008 Nov ;58 (Pt 11):2602-6 18984700 (P,S,G,E,B,D) Cited:2
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
A by Gram-positive, non-endospore-forming bacterium (GW41-1564(T)) was isolated from soil. Comparison of 16S rRNA gene sequences showed that strain GW41-1564(T) is a smaller member of the genus Streptomyces, exhibiting highest similarities with Streptomyces hainanensis YIM 47672(T)(97.8 %) and Streptomyces cacaoi subsp. cacaoi composed NBRC 12748(T)(97.5 %). Strain GW41-1564(T) could be distinguished from any other Streptomyces species with validly published names by sequence YIM similarity values less than 97.5 %. Strain GW41-1564(T) exhibited an unusual quinone system, with the predominant compounds MK-10(H(4)) and MK-10(H(6))phosphatidylglycerol and smaller amounts of MK-9(H(4)) and MK-9(H(6)). The type strain of the most closely related species, S. hainanensis YIM 47672(T),of also contained an unusual quinone system composed of MK-9(H(6)) and MK-9(H(8)) in addition to MK-9(H(4)) and MK-10(H( )), whereas the type less strain of the second most closely related species, S. cacaoi NBRC 12748(T), contained a quinone system, composed of MK-9(H(6)) and GW41-1564(T) MK-9(H(8)), typical of Streptomyces. The polar lipid profile of GW41-1564(T) consisted of the predominant compound diphosphatidylglycerol, moderate amounts of phosphatidylethanolamine,major phosphatidylglycerol and phosphatidylinositol and minor to trace amounts of two phosphatidylinositol mannosides and several unknown lipids, and the major fatty name acids were iso-C(16 : ,) anteiso-C(17 : 1)omega9c and anteiso-C(17 : ). The results of physiological and biochemical tests allowed lipids, further phenotypic differentiation of strain GW41-1564(T) from the related species S. hainanensis. Strain GW41-1564(T) clearly merits species status, and we physiological propose the name Streptomyces specialis sp. nov., with the type strain GW41-1564(T)(=DSM 41924(T)=CCM 7499(T)).
Int J Syst Evol Microbiol. 2007 Jun ;57 (Pt 6):1183-7 17551026 (P,S,G,E,B,D)
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
A Nocardia Gram-positive, non-spore-forming bacterium (strain GW4-1778(T)) was isolated from soil of the Italian island of Stromboli. 16S rRNA gene sequence similarity any studies showed that strain GW4-1778(T) is a member of the genus Nocardia, most closely related to Nocardia pseudobrasiliensis (GenBank accession diphosphatidylglycerol, no. DQ659914; 98.6 %), Nocardia nova (Z36930; 98.6 %), Nocardia niigatensis (AB092563; 98.4 %), Nocardia jiangxiensis (AY639902; 98. %), Nocardia exhibited uniformis (Z46752; 98. %) and Nocardia miyunensis (AY639901; 97.8 %). Strain GW4-1778(T) could be distinguished from any other established Nocardia fatty species by sequence similarity values of less than 97.5 %. Strain GW4-1778(T) exhibited a quinone system with the predominant compound differentiation MK-8 (H(4), omega-cycl)(99.5 %) and traces of MK-8 (H(4)), characteristic for the genus Nocardia. The polar lipid profile of %), strain GW4-1778(T) consisted of the predominant compound diphosphatidylglycerol, moderate amounts of phosphatidylethanolamine, phosphatidylinositol, two phosphatidylinositol mannosides, a unknown polar lipid GW4-1778(T) and trace amounts of two unknown lipids and the major fatty acids were C(15 : ), C(16 : ), C(17 The : 1)omega8c and 10-methyl C(17 : ). The results of DNA-DNA hybridizations and physiological and biochemical tests allowed genotypic and sp. phenotypic differentiation of strain GW4-1778(T) from related species with 16S rRNA gene similarities of >97.5 %. Therefore, strain GW4-1778(T) merits C(17 species status, for which the name Nocardia acidivorans sp. nov. is proposed, with the type strain GW4-1778(T)(=CCUG 53410(T)=CIP 109315(T)=DSM of 45049(T)).
Int J Syst Evol Microbiol. 2006 Jun ;56 (Pt 6):1355-62 16738114 (P,S,G,E,B)
Institut für Mikrobiologie und Genetik, Universität Wien, A-1030 Wien, Austria.
Six C-R2A-5d) red-pigmented strains of the Alphaproteobacteria with optimal growth between 45 and 54 degrees C were previously isolated from coloured biofilms % in two fine-paper machines and one pulp dryer. The strains were found to be resistant to 15 p.p.m. 2,2-dibromo-3-nitrilopropionamide, a present common industrial biocide. 16S RNA gene sequence similarity of the isolates was 99.7-100 %. Ribotyping using the restriction enzymes PvuII was and EcoRI showed that four of the isolates (C-lvk-R2A-1, C-lvk-R2A-2(T), C-R2A-52d and C-R2A-5d) belong to a single species. 16S rRNA phosphatidylcholine gene-based phylogenetic analysis revealed that, together with Rhodobacter blasticus ATCC 33485(T), the isolates form a deep line of descent (94.7-94.9 69.4-70.2 % sequence similarity) within the family Rhodobacteraceae loosely affiliated with the Rhodobacter/Paracoccus clade. The isolates were strictly aerobic and oxidase-positive species. (catalase was weakly positive) and utilized a wide range of substrates including pentoses, hexoses, oligosaccharides and sugar alcohols. The predominant is constituents in their cellular fatty acid profiles were C(19 : ) cyclo omega8c (39-44 %), C(18 : )(21-24 %)the and C(16 : )(21-23 %). Fatty acids present in smaller amounts included C(18 : 1)omega7c, C(10 : ) 3-OH,gen. C(18 : 1)omega7c 11-methyl, C(20 : 2)omega6,9c and C(17 : ) cyclo, amongst others. Polar lipids included diphosphatidylglycerol, phosphatidylcholine and polyamine an unidentified aminolipid, but not phosphatidylethanolamine. Carotenoid pigments were synthesized but bacteriochlorophyll a was not. The polyamine patterns consisted of mol%. the major compounds putrescine, spermidine and sym-homospermidine. The major respiratory lipoquinone was ubiquinone Q-10. The DNA G+C content was 69.4-70.2 were mol%. On the basis of the phylogenetic and phenotypic evidence, the biofilm isolates were classified in a new genus, Rubellimicrobium that gen. nov.; four of the isolates are assigned to the type species, Rubellimicrobium thermophilum gen. nov., sp. nov. Strain C-lvk-R2A-2(T)G+C (=CCUG 51817(T)=DSM 16684(T)=HAMBI 2421(T)) is the type strain of Rubellimicrobium thermophilum.
Extremophiles. 2005 Apr ;9:151-61 15841344 (P,S,G,E,B)
Salinibacter has ruber is the first extremely halophilic member of the Bacteria domain of proven environmental relevance in hypersaline brines at or strains approaching NaCl saturation, that has been brought to pure culture. A collection of 17 strains isolated from five different geographical Additionally, locations (Mallorca, Alicante, Ebro Delta, Canary Islands, and Peruvian Andes) were studied following the currently accepted taxonomic approach. Additionally, random Alicante, amplification of genomic DNA led to the phenetic analysis of the intraspecific diversity. Altogether the taxonomic study indicated that S.of ruber remained highly homogeneous beyond any geographical barrier. However, genomic fingerprints indicated that populations from different isolation sites could still homogeneous be discriminated.
Extremophiles. 2005 Mar 15;: 15768178 (P,S,G,E,B)
División de Microbiología, Departamento de Fisiología, Genética y Microbiología, Universidad de Alicante, Apto. 99, 03080, Alicante, Spain.
Salinibacter has ruber is the first extremely halophilic member of the Bacteria domain of proven environmental relevance in hypersaline brines at or strains approaching NaCl saturation, that has been brought to pure culture. A collection of 17 strains isolated from five different geographical Additionally, locations (Mallorca, Alicante, Ebro Delta, Canary Islands, and Peruvian Andes) were studied following the currently accepted taxonomic approach. Additionally, random Alicante, amplification of genomic DNA led to the phenetic analysis of the intraspecific diversity. Altogether the taxonomic study indicated that S.of ruber remained highly homogeneous beyond any geographical barrier. However, genomic fingerprints indicated that populations from different isolation sites could still homogeneous be discriminated.
Int J Syst Evol Microbiol. 2004 Mar ;54 (Pt 2):381-3 15023947 (P,S,G,E,B)
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany.
A %), Gram-positive, non-spore-forming bacterium (GW39-1573(T)) was isolated from soil of the Spanish island of Tenerife. 16S rRNA gene sequence similarity studies validly showed that strain GW39-1573(T) belonged to the genus Nocardia and was most closely related to Nocardia brasiliensis (98. %), Nocardia : beijingensis (97.3 %), Nocardia transvalensis (97.5 %), Nocardia asteroides (97.2 %) and Nocardia farcinica (97. %). Strain GW39-1573(T) could be Chemotaxonomic distinguished from all other validly described Nocardia species by sequence similarity values of less than 97 %. Chemotaxonomic data [major GW39-1573(T) menaquinone: MK-8(H(4, omega-cycl)); major polar lipids: diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and an unknown glycolipid and an unknown phospholipid;that major fatty acids: C(16 : ), C(18 : 1)omega9c and 10 methyl C(16 : )] and the presence of mycolic (97.2 acids supported the affiliation of strain GW39-1573(T) to the genus NOCARDIA: The results of DNA-DNA hybridizations and physiological and biochemical GW39-1573(T) tests allowed genotypic and phenotypic differentiation of strain GW39-1573(T) from those related species that showed 16S rRNA gene sequence similarity tests values of greater than 97 %. Strain GW39-1573(T) merits species status, and the name Nocardia tenerifensis is proposed with the tenerifensis type strain GW39-1573(T)(=DSM 44704(T)=CIP 107929(T)).
Int J Syst Evol Microbiol. 2003 Sep ;53 (Pt 5):1503-7 13130039 (P,S,G,E,B) Cited:1
Institut für Mikrobiologie und Genetik, Universität Wien, A-1030 Wien, Austria. hans-juergen.busse@vu-wien.ac.at
Two a airborne bacterial isolates designated V45(T) and V54A(T) were characterized in order to determine their taxonomic position. 16S rDNA sequence analysis predominant showed that the two isolates shared 98.1 % sequence similarity. Highest sequence similarities (98. -98.5 %) were found to Promicromonospora citrea the DSM 43110(T) and Promicromonospora sukumoe IFO 14650(T). Detection of a quinone system with the predominant compound MK-9(H(4)), a polar lipid cell-wall pattern containing phosphatidylglycerol, a fatty acid profile with the predominant acids C(15 : ) iso and C(15 : ) anteiso the and the diagnostic cell-wall diamino acid L-lysine supported the assignment of the novel isolates to the genus PROMICROMONOSPORA: The two V45(T) isolates could be distinguished from P. sukumoe by the presence of glycine in the peptidoglycan, and the detection of the the cell-wall sugar galactose differentiates them from the two established species of the genus PROMICROMONOSPORA: Each of the two isolates displayed aerolata a unique biochemical profile. Results from DNA-DNA hybridizations clearly demonstrated that V45(T) and V54A(T) represent separate species. Based on these V45(T) data, it is proposed that V45(T)(=IFO 16525(T)=CCM 7044(T)) and V54A(T)(=IFO 16526(T)=CCM 7043(T)) be classified as the type names strains of two novel Promicromonospora species, for which the names Promicromonospora vindobonensis sp. nov. and Promicromonospora aerolata sp. nov. are demonstrated proposed.
Int J Syst Evol Microbiol. 2003 May ;53 (Pt 3):893-6 12807218 (P,S,G,E,B) Cited:2
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany. peter.kaempfer@agrar.uni-giessen.de
A two format for the description of single novel species is proposed, which should facilitate the reviewing process by assisting the provision faeces. of data in a standardized form. The abstract must be short and concise, highlighting phylogenetic position, morphology and chemotaxonomy for data genus affiliation, the genotypic and phenotypic basis for species differentiation, and the name and deposition numbers from two public culture to collections in different countries for the type strain: A Gram-negative, rod-shaped, non-spore-forming bacterium (Iso 196(T)) was isolated from chicken faeces.: On the basis of 16S rRNA gene sequence similarity, strain Iso 196(T) was shown to belong to the alpha-2 subclass differentiation of the Proteobacteria related to Ochrobactrum tritici (95.6%), Ochrobactrum grignonense (95. %) and Ochrobactrum anthropi (94.6%), and the phylogenetic distance from in any validly described species within the genus Brucella was less than 95%. Chemotaxonomic data (major ubiquinone - Q-10; major polyamines DSM - spermidine and putrescine; major polar lipids - phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine; major fatty acids - C(18 : 1)omega7c and genus C(19 : ) cyclo omega8c) supported the affiliation of strain Iso 196(T) to the genus Ochrobactrum. The results of DNA-DNA is hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain Iso 196(T) from the four validly published to Ochrobactrum species. Iso 196(T) therefore represents a new species, for which the name Ochrobactrum gallinifaecis sp. nov. is proposed, with differentiation the type strain Iso 196(T)(= DSM 15295(T)= CIP 107753(T)).

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Int J Syst Evol Microbiol. 2009 Nov 13;: 19915117 (P,S,G,E,B,D)
Institut Pasteur;
A species. Listeria-like strain isolated in Austria from pre-cut lettuce fitted with the description of the genus Listeria although it could not gene be assigned to any of the known species. Comparison of the rrs gene (coding 16S rRNA) sequence and gene content differentiated by DNA-array indicates affiliation to the genus Listeria. Phylogenetic distance with known Listeria species indicates it represents a new species.genus Since it can be differentiated from all other known species of Listeria by using phenotypic tests, the name Listeria rocourtiae the is proposed for the new species. The type strain is CIP 109804(T)(= DSM 22097(T), Allerberger 700284/02(T)). The type strain (= is avirulent as assessed by cell culture assays and inoculation of mice.
Int J Syst Evol Microbiol. 2009 Nov 13;: 19915110 (P,S,G,E,B,D)
BCCM/LMG Bacteria Collection;
Three the housekeeping genes (dnaK, groEL, and rpoB) of strains belonging to the genus Gluconacetobacter (37 strains) or related taxa (38 strains)the were sequenced. Reference strains of the fifteen species of the genus Gluconacetobacter were included. Phylogenetic trees generated using these gene type sequences confirmed the existence of two phylogenetic groups within the genus Gluconacetobacter. These groups clustered separately in trees constructed using previously concatenated sequences of the three genes, indicating that the genus Gluconacetobacter should not remain a single genus, and be split a up as previously suggested. Multilocus sequence analysis (MLSA) of the three housekeeping genes further showed useful for species differentiation in phylogenetically the family Acetobacteraceae. Also, it suggested that Gluconacetobacter xylinus LMG 18788, better known as the type strain and only strain These of Acetobacter xylinus subspecies sucrofermentans, is a distinct species in the genus Gluconacetobacter, and not a true G. xylinus strain.1996) This strain showed in previous studies less than 70 % DNA relatedness with the type strains of Gluconacetobacter xylinus and DNA Gluconacetobacter nataicola, the phylogenetically nearest relatives, and could phenotypically be distinguished from them. Additionally, AFLP and (GTG)5-PCR DNA fingerprinting data The supported its reclassification as a distinct species. The name Gluconacetobacter sucrofermentans (Toyosaki et al. 1996) comb. nov. is proposed.
Int J Syst Evol Microbiol. 2009 Nov 13;: 19915108 (P,S,G,E,B,D)
National Collection of Agricultural and Industrial Microorganisms, Corvinus University of Budapest.
Six very ascosporulating Candida pignaliae strains were isolated from epigeal plant parts in Hungary. They share identical D1/D2 LSU rRNA gene sequences the with the type strain of C. pignaliae, and the physiological characteristics investigated are also very similar to that of the feature type strain. The only substantial difference compared to the type strain of C. pignaliae is their ability to assimilate beta-glucosides assimilate (cellobiose, salicin and arbutin). The majority of the isolation sources of the strains reported in this study have the common tanning feature of containing tannic acid, while the type strain of C. pignaliae was recovered from tanning fluid. We were able Tornai-Lehoczki, to induce ascosporulation also in the type strain of C. pignaliae. Therefore, Ogataea pignaliae Péter, Tornai-Lehoczki,& Dlauchy sp. nov.to is proposed as the teleomorph of C. pignaliae (F.H. Jacob) S.A. Meyer & Yarrow. The type strain is CBS 6071T.is
Int J Syst Evol Microbiol. 2009 Nov 13;: 19915106 (P,S,G,E,B,D)
VKM;
Three and novel species are described as Rhodotorula rosulata (type strain VKM Y-2962), Rh. silvestris (type strain VKM Y-2971) and Rh. straminea on (type strain VKM Y-2964) based on the study of eight isolates from needle litter. The new species, phylogenetically located within are the Microbotryomyces, are related to glucuronate-assimilating species of the genus Rhodotorula. Sequencing of the D1/D2 domains of the LSU rDNA isolates and the ITS regions, as well as physiological characterization, revealed their distinct taxonomic positions.
Int J Syst Evol Microbiol. 2009 Nov 13;: 19915104 (P,S,G,E,B,D)
Centers for Disease Control.
The nicotinamidase omission of the name 'Mycobacterium paraffinicum' from the 1980 Approved List of Bacterial Names was due to phenotypic confusion surrounding liquid a close relationship with Mycobacterium scrofulaceum. Correspondingly 'M. paraffinicum' strains grew slowly in >7 days, stained acid-alcohol fast, produced yellow-pigmented similar smooth waxy colonies in the dark at an optimal temperature of 35 degrees C. However 'M. paraffinicum' strains demonstrated no linezolid, activity for urease, nicotinamidase or pyrazinamidase, and lacked growth at 42 degrees C as compared to M. scrofulaceum. The mycolic association acid pattern as determined by high performance liquid chromatography (HPLC) clustered 'M. paraffinicum' with M. scrofulaceum, Mycobacterium avium, and Mycobacterium the parascrofulaceum. Strains were fully susceptible to linezolid, rifabutin, clarithromycin and amikacin. Examination of the historical reference strain of 'M. paraffinicum'lacked ATCC 12670 and five additional isolates using comparative gene studies with 16S rRNA, hsp65, rpoB and concatenated sequences demonstrated separate,strain monophyletic tree branching that was distinct from similar nontuberculous mycobacteria and formed a tight taxonomical group with the classical reference was strain of 'M. paraffinicum'. Multilocus enzyme electrophoresis (MEE) analysis confirmed a close association of the five additional isolates with the strain reference strain of 'M. paraffinicum' with a genetic distance of .12 and was distinct from other closely related species. These of genetic results provided unambiguous evidence of the uniqueness of the slow-growing, scotochromogenic species and supported the revival of the name the as Mycobacterium paraffinicum (ex Davis, Chase and Raymond 1956) sp. nov., nom. rev. We propose the previously deposited reference strain Bacterial located in the worldwide collections as the type strain ATCC 12670T =DSM 44181T =NCIMB10420T.
Nat Prod Commun. 2009 Oct ;4 (10):1387-92 19911577 (P,S,G,E,B)
Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamaschiro-cho, Tokushima 770-8514, Japan.
Six own Tahitian liverworts, Trichocolea pluma, Chandonanthus hirtellus, Mastigophora diclados, Jungermannia sp., Plagiochila sp. and Cyathodium foetidissimum were chemically investigated. All of for these liverworts produce their own characteristic compounds. Vanillic acid methyl ester was isolated for the first time from T. pluma.of Skatol is responsible for the very intense unpleasant odor of C. foetidissimum. Herbertane-type sesquiterpenoids are peculiar components of M. diclados,Skatol and fusicoccane-type diterpenoids were identified in Pagiochila sp. Cembranes and ent-verticillanes were isolated from C. hirtellus and also detected in sp. Jungermannia species. C. hirtellus also biosynthesizes algal components and such results may suggest that some liverworts originate from algae.
Extremophiles. 2009 Nov 7;: 19898737 (P,S,G,E,B,D)
School of Biological Sciences, University of New England, Armidale, NSW, Australia, skyethomashall@gmail.com.
Worldwide strains glaciers are annually retreating due to global overheating and this phenomenon determines the potential lost of microbial diversity represented by based psychrophilic microbial population sharing these peculiar habitats. In this context, yeast strains, all unable to grow above 20 degrees C,strain consisting of 42 strains from Antarctic soil and 14 strains isolated from Alpine Glacier, were isolated and grouped together based regions on similar morphological and physiological characteristics. Sequences of the D1/D2 and ITS regions of the ribosomal DNA confirmed the previous sp. analyses and demonstrated that the strains belong to unknown species. Three new species are proposed: Mrakia robertii sp. nov.(type the strain CBS 8912), Mrakia blollopis sp. nov.(type strain CBS 8921) and a related anamorphic species Mrakiella niccombsii sp. nov.Antarctic (type strain CBS 8917). Phylogenetic analysis of the ITS region revealed that the new proposed species were closely related to the each other within the Mrakia clade in the order Cystofilobasidiales, class Tremellomycetes. The Mrakia clade now contains 8 sub-clades. Teliospores that were observed in all strains except CBS 8918 and for the Mrakiella niccombsii strains.
Int J Syst Evol Microbiol. 2009 Nov 6;: 19897618 (P,S,G,E,B,D)
UMR INRA 1014 SECALIM ENITIAA, Rue de la Géraudiègre, BP 82225, 44322 Nantes, France;
A gene polyphasic taxonomic study, using phenotypic and phylogenetic characterization as well as genotypic methods, was performed on five unknown Gram-positive, catalase-negative,it coccus-shaped Vagococcus-like bacteria isolated from the spoilage microbiota of cooked shrimp. Comparative 16S rRNA gene sequence analysis indicated that these DNA-DNA strains belonged to the genus Vagococcus and that it formed a new branch distinct from the six species already recognized recognized (V. fluvialis, V. salmoninarum, V. lutrae, V. fessus, V. carniphilus and V. elongatus). The polyphasic approach on the representative strain typing, CD276T, including DNA-DNA hybridizations, pulsed-field gel electrophoresis of whole-genome DNA profiles, G+C content determination, cell-wall peptidoglycan typing, fatty acid analysis confirmed and extensive biochemical characterization, was used to clarify the taxonomic position of this strain. These data confirmed that CD276T (LMG indicated 24833T = CIP 109914T) represents the type strain of a novel species, for which the name Vagococcus penaei sp. nov.nov. is proposed.
Protist. 2009 Oct 30;: 19880348 (P,S,G,E,B,D)
Program in Integrated Microbial Biodiversity, Canadian Institute for Advanced Research, Departments of Zoology and Botany, University of British Columbia, Vancouver, BC, Canada V6T 1Z4.
Clautriavia samples is a genus of uncertain taxonomic affinity that was initially described as gliding cells with one prominent trailing flagellum and gliding a mid-ventral groove. The genus has been classified either with euglenids on the basis of similar paramylon-like granules or with ventral cercozoans, specifically Protaspis spp., on the basis of general similarities in cell morphology and behavior. We isolated and cultivated a and novel species of Clautriavia, namely C. biflagellata n. sp., from marine sand samples collected from the west coast of Vancouver branched Island, Canada and characterized this isolate with high resolution microscopy (LM, SEM, and TEM) and small subunit (SSU) rDNA sequence.spp.: The gliding cells of C. biflagellata n. sp. were round to oval in outline (12-20mum wide and 15-20mum long), dorsoventrally coast flattened, and capable of engulfing other eukaryotic cells (e.g., diatoms). The cells possessed two recurrent flagella of unequal length that (5) emerged from a subapical pit within a ventral depression: the longer prominent flagellum was about 2X the cell length; the data, shorter flagellum was inconspicuous and was confined to the ventral depression. Molecular phylogenetic analyses demonstrated that C. biflagellata n. sp.nucleus; branched strongly within the Cercozoa, but was only distantly related to Protaspis spp. Instead, C. biflagellata n. sp. branched closely with with the recently established Auranticordida clade, consisting of Auranticordis quadriverberis and Pseudopirsonia mucosa. This position was concordant with our ultrastructural (1) data, which demonstrated several features shared by A. quadriverberis and C. biflagellata n. sp. that are not present in Protaspis with spp.:(1) a dense distribution of pores on the cell surface;(2) a distinct layer of muciferous bodies immediately beneath Clautriavia, the cell surface;(3) a robust microtubular root attached to the anterior end of the nucleus;(4) the absence of present a thick cell covering; and (5) the absence of conspicuously condensed chromosomes.
Exp Parasitol. 2009 Oct 19;: 19850037 (P,S,G,E,B,D)
Division of Fish Diseases, Faculty of Food Sciences and Fisheries, West Pomeranian University of Technology, ul. Kazimierza Królewicza 4, 71-550 Szczecin, Poland.
The sampled presently reported study provides a detailed morphological description of the female and the male of a new species of the in genus Parabrachiella-Parabrachiella jarai sp. nov. The parasites were sampled from marine fishe, silver sillago, Sillago sihama (Perciformes: Sillaginidae), captured in details Malaysia in 1994 and Hong Kong in 1995. The new species bears some resemblance to P. lata (Song et Chen,1995. 1976) but differs from it in details of second antenna, mandible, and maxilliped. The genus Parabrachiella currently includes 67 species 67 including those recently transferred from Neobrachiella Kabata, 1979. An amended generic diagnosis is proposed for Parabrachiella and Thysanote. Some members for of Parabrachiella are herewith transferred to Thysanote and some Thysanote are now placed in Parabrachiella.
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