Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, Veterinärplatz 1, A-1210 Vienna, Austria.
Two airborne bacterial isolates, NS/2 and NS/50(T), were examined in order to determine their taxonomic position. Their almost complete 16S rRNA gene sequences shared 95.9 % similarity. Sequence comparisons demonstrated that their next relatives are species of the genus Hymenobacter (93.6-95.7 % similarity) and the strains 'Taxeobacter chitinovorans' Txc1(T),'Taxeobacter gelupurpurascens' Txg1(T) and 'Taxeobacter ocellatus' Myx 2105(T)(90.5-96.4 %). Phylogenetic calculations indicated that these five strains together with the three recognized Hymenobacter species form a separate line of descent within the family 'Flexibacteraceae'. Isolates NS/2 and NS/50(T), as well as 'Taxeobacter chitinovorans' Txc1(T),'Taxeobacter gelupurpurascens' Txg1(T) and 'Taxeobacter ocellatus' Myx 2105(T), possessed the characteristics of the genus Hymenobacter, the quinone system menaquinone MK-7 and a polyamine pattern with the major polyamine being sym-homospermidine. Each of the five strains had complex, unique polar lipid profiles, with phosphatidylethanolamine and several unknown aminophospho-, amino-, phospho-, glyco- and polar lipids of which several compounds were also found in established Hymenobacter species. All the strains studied possessed fatty acids characteristic of Hymenobacter species, including major acids iso-C(15 : 0), anteiso-C(15 : 0), C(16 : 1)omega5c, summed feature 3 (C(16 : 1)omega7c/iso-C(15 : 0) 2-OH) and summed feature 4 (iso-C(17 : 1) I/anteiso-C(17 : 1) B). The five strains could be distinguished from each other and from the three established species of the genus Hymenobacter based on relatively low 16S rRNA gene sequence similarities (<97 %), unique polar lipids and differing fatty acid profiles and physiological characteristics. In conclusion, the description of four novel species of the genus Hymenobacter appears to be justified, for which the names Hymenobacter norwichensis sp. nov.(type strain NS/50(T)=LMG 21876(T)=DSM 15439(T)), Hymenobacter chitinivorans sp. nov.(type strain Txc1(T)=LMG 21951(T)=DSM 11115(T)), Hymenobacter gelipurpurascens sp. nov.(type strain Txg1(T)=LMG 21873(T)=DSM 11116(T)) and Hymenobacter ocellatus sp. nov.(type strain Myx 2105(T)=Txo1(T)=LMG 21873(T)=DSM 11117(T)) are proposed. For strain NS/2, a description only is provided without proposal of a name because its status as a novel species was not demonstrated unambiguously.
Other papers by authors:
Hans-Jürgen Busse,
Ewald B M Denner,
Sandra Buczolits,
Mirja Salkinoja-Salonen,
Antonio Bennasar,
Peter Kämpfer
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, A-1210 Vienna, Austria. hans-juergen.busse@vu-wien.ac.at
Seven psychrotolerant, Gram-negative bacterial strains, five dust- and airborne isolates (MA101b(T), MA306a, MA405/90, MA-olki(T) and NW12(T)) and two from the Antarctic (Ant 20 and M3C203B-B), were subjected to a polyphasic characterization to determine their taxonomic position. High 16S rDNA sequences similarities (99.3-100.0 %) demonstrated that they were closely related to each other. Phylogenetic evaluation of their 16S rDNA sequences revealed that they are members of the genus Sphingomonas sensu stricto, encompassing a separate branch within this genus. They shared 94.4-96.6 % 16S rDNA sequence similarity with species of this genus. All Sphingomonas-specific signature nucleotides were also detected. The presence of the major ubiquinone Q-10, sym-homospermidine as the predominant polyamine, Sphingomonadaceae-specific sphingoglycolipid in the polar lipid patterns and a fatty acid profile containing C(14 : 0) 2-OH and lacking 3-OH fatty acids were in agreement with identification of these strains as members of the genus Sphingomonas sensu stricto. Results from DNA-DNA hybridizations and comparison of protein patterns indicated that the seven strains are members of three distinct species. One species is represented by strains MA101b(T), MA306a and MA405/90, the second by strains NW12(T), Ant 20 and M3C203B-B and the third by one strain, MA-olki(T). Their distinction at the species level was also supported by results of biochemical characterization and partly supported by riboprints and genomic fingerprints. On the basis of these results, three novel species of the genus Sphingomonas are proposed: Sphingomonas aurantiaca sp. nov., consisting of strains MA101b(T)(=DSM 14748(T)=LMG 21377(T)), MA306a and MA405/90 (=DSM 14749=LMG 21378), Sphingomonas faeni sp. nov. MA-olki(T)(=DSM 14747(T)=LMG 21379(T)) and Sphingomonas aerolata sp. nov., represented by strains NW12(T)(=DSM 14746(T)=LMG 21376(T)), Ant 20 (=ICMP 13599) and M3C203B-B (=SMCC M3C203B-B).
Sandra Buczolits,
Ewald B M Denner,
Dietmar Vybiral,
Monika Wieser,
Peter Kämpfer,
Hans-Jürgen Busse
Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, Wien, Austria.
Three aerobic, gram-negative, rod-shaped, non-spore-forming, red-pigmented, airborne bacteria (I/26-Cor1T, I/32A-Cor1 and I/74-Cor2) collected in the Museo Correr (Venice, Italy) were investigated to determine their taxonomic status by analysing their biochemical, physiological and chemotaxonomic features and the G+C content of genomic DNA and by comparing their genomic fingerprints. Additionally, the almost complete 16S rRNA gene sequence of strain I/26-Cor1T was analysed. The three strains were nearly identical in their morphological, physiological, biochemical and chemotaxonomic properties. The strains contained a menaquinone system with the predominant menaquinone MK-7 and a fatty acid profile with C15:0 anteiso, C15:0 iso and C16:1 predominant. Phosphatidylethanolamine and several unidentified lipids were detected in the polar lipid profiles. The polyamine pattern consisted of sym-homospermidine as the major compound. meso-Diaminopimelic acid was found as the characteristic cell-wall diamino acid. The DNA base composition of the three strains ranged from 60 to 63 mol% G+C. Phylogenetically, strain I/26-Cor1T was most closely related to Hymenobacter actinosclerus (95.8% 16S rRNA gene sequence similarity). Physiological and genomic characteristics indicated that the two strains I/26-Cor1T and I/32A-Cor1 are representatives of the same species. The phylogenetic distance to any validly described taxon as indicated by 16S rRNA gene sequence similarities demonstrates that I/26-Cor1T and I/32A-Cor1 represent a novel species, for which the name Hymenobacter aerophilus sp. nov. is proposed, with the type strain I/26-Cor1T (= DSM 13606T = LMG 19657T). I/32A-Cor1 (= DSM 13607 = LMG 19658) is another strain of the species Hymenobacter aerophilus. Since the taxonomic status of strain I/74-Cor2 within the genus Hymenobacter was not determined unambiguously, it is designated Hymenobacter sp. I/74-Cor2 (= DSM 13611 = LMG 19659).
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
A Gram-positive, non-endospore-forming bacterium (GW41-1564(T)) was isolated from soil. Comparison of 16S rRNA gene sequences showed that strain GW41-1564(T) is a member of the genus Streptomyces, exhibiting highest similarities with Streptomyces hainanensis YIM 47672(T)(97.8 %) and Streptomyces cacaoi subsp. cacaoi NBRC 12748(T)(97.5 %). Strain GW41-1564(T) could be distinguished from any other Streptomyces species with validly published names by sequence similarity values less than 97.5 %. Strain GW41-1564(T) exhibited an unusual quinone system, with the predominant compounds MK-10(H(4)) and MK-10(H(6)) and smaller amounts of MK-9(H(4)) and MK-9(H(6)). The type strain of the most closely related species, S. hainanensis YIM 47672(T), also contained an unusual quinone system composed of MK-9(H(6)) and MK-9(H(8)) in addition to MK-9(H(4)) and MK-10(H(0)), whereas the type strain of the second most closely related species, S. cacaoi NBRC 12748(T), contained a quinone system, composed of MK-9(H(6)) and MK-9(H(8)), typical of Streptomyces. The polar lipid profile of GW41-1564(T) consisted of the predominant compound diphosphatidylglycerol, moderate amounts of phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol and minor to trace amounts of two phosphatidylinositol mannosides and several unknown lipids, and the major fatty acids were iso-C(16 : 0,) anteiso-C(17 : 1)omega9c and anteiso-C(17 : 0). The results of physiological and biochemical tests allowed further phenotypic differentiation of strain GW41-1564(T) from the related species S. hainanensis. Strain GW41-1564(T) clearly merits species status, and we propose the name Streptomyces specialis sp. nov., with the type strain GW41-1564(T)(=DSM 41924(T)=CCM 7499(T)).
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
A Gram-positive, non-spore-forming bacterium (strain GW4-1778(T)) was isolated from soil of the Italian island of Stromboli. 16S rRNA gene sequence similarity studies showed that strain GW4-1778(T) is a member of the genus Nocardia, most closely related to Nocardia pseudobrasiliensis (GenBank accession no. DQ659914; 98.6 %), Nocardia nova (Z36930; 98.6 %), Nocardia niigatensis (AB092563; 98.4 %), Nocardia jiangxiensis (AY639902; 98.0 %), Nocardia uniformis (Z46752; 98.0 %) and Nocardia miyunensis (AY639901; 97.8 %). Strain GW4-1778(T) could be distinguished from any other established Nocardia species by sequence similarity values of less than 97.5 %. Strain GW4-1778(T) exhibited a quinone system with the predominant compound MK-8 (H(4), omega-cycl)(99.5 %) and traces of MK-8 (H(4)), characteristic for the genus Nocardia. The polar lipid profile of strain GW4-1778(T) consisted of the predominant compound diphosphatidylglycerol, moderate amounts of phosphatidylethanolamine, phosphatidylinositol, two phosphatidylinositol mannosides, a unknown polar lipid and trace amounts of two unknown lipids and the major fatty acids were C(15 : 0), C(16 : 0), C(17 : 1)omega8c and 10-methyl C(17 : 0). The results of DNA-DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain GW4-1778(T) from related species with 16S rRNA gene similarities of >97.5 %. Therefore, strain GW4-1778(T) merits species status, for which the name Nocardia acidivorans sp. nov. is proposed, with the type strain GW4-1778(T)(=CCUG 53410(T)=CIP 109315(T)=DSM 45049(T)).
Ewald B M Denner,
Marko Kolari,
Douwe Hoornstra,
Irina Tsitko,
Peter Kämpfer,
Hans-Jürgen Busse,
Mirja Salkinoja-Salonen
Institut für Mikrobiologie und Genetik, Universität Wien, A-1030 Wien, Austria.
Six red-pigmented strains of the Alphaproteobacteria with optimal growth between 45 and 54 degrees C were previously isolated from coloured biofilms in two fine-paper machines and one pulp dryer. The strains were found to be resistant to 15 p.p.m. 2,2-dibromo-3-nitrilopropionamide, a common industrial biocide. 16S RNA gene sequence similarity of the isolates was 99.7-100 %. Ribotyping using the restriction enzymes PvuII and EcoRI showed that four of the isolates (C-lvk-R2A-1, C-lvk-R2A-2(T), C-R2A-52d and C-R2A-5d) belong to a single species. 16S rRNA gene-based phylogenetic analysis revealed that, together with Rhodobacter blasticus ATCC 33485(T), the isolates form a deep line of descent (94.7-94.9 % sequence similarity) within the family Rhodobacteraceae loosely affiliated with the Rhodobacter/Paracoccus clade. The isolates were strictly aerobic and oxidase-positive (catalase was weakly positive) and utilized a wide range of substrates including pentoses, hexoses, oligosaccharides and sugar alcohols. The predominant constituents in their cellular fatty acid profiles were C(19 : 0) cyclo omega8c (39-44 %), C(18 : 0)(21-24 %) and C(16 : 0)(21-23 %). Fatty acids present in smaller amounts included C(18 : 1)omega7c, C(10 : 0) 3-OH, C(18 : 1)omega7c 11-methyl, C(20 : 2)omega6,9c and C(17 : 0) cyclo, amongst others. Polar lipids included diphosphatidylglycerol, phosphatidylcholine and an unidentified aminolipid, but not phosphatidylethanolamine. Carotenoid pigments were synthesized but bacteriochlorophyll a was not. The polyamine patterns consisted of the major compounds putrescine, spermidine and sym-homospermidine. The major respiratory lipoquinone was ubiquinone Q-10. The DNA G+C content was 69.4-70.2 mol%. On the basis of the phylogenetic and phenotypic evidence, the biofilm isolates were classified in a new genus, Rubellimicrobium gen. nov.; four of the isolates are assigned to the type species, Rubellimicrobium thermophilum gen. nov., sp. nov. Strain C-lvk-R2A-2(T)(=CCUG 51817(T)=DSM 16684(T)=HAMBI 2421(T)) is the type strain of Rubellimicrobium thermophilum.
Arantxa Peña,
Maria Valens,
Fernando Santos,
Sandra Buczolits,
Josefa Antón,
Peter Kämpfer,
Hans-Jürgen Busse,
Rudolf Amann,
Ramon Rosselló-Mora
Salinibacter ruber is the first extremely halophilic member of the Bacteria domain of proven environmental relevance in hypersaline brines at or approaching NaCl saturation, that has been brought to pure culture. A collection of 17 strains isolated from five different geographical locations (Mallorca, Alicante, Ebro Delta, Canary Islands, and Peruvian Andes) were studied following the currently accepted taxonomic approach. Additionally, random amplification of genomic DNA led to the phenetic analysis of the intraspecific diversity. Altogether the taxonomic study indicated that S. ruber remained highly homogeneous beyond any geographical barrier. However, genomic fingerprints indicated that populations from different isolation sites could still be discriminated.
Arantxa Peña,
Maria Valens,
Fernando Santos,
Sandra Buczolits,
Josefa Antón,
Peter Kämpfer,
Hans-Jürgen Busse,
Rudolf Amann,
Ramon Rosselló-Mora
División de Microbiología, Departamento de Fisiología, Genética y Microbiología, Universidad de Alicante, Apto. 99, 03080, Alicante, Spain.
Salinibacter ruber is the first extremely halophilic member of the Bacteria domain of proven environmental relevance in hypersaline brines at or approaching NaCl saturation, that has been brought to pure culture. A collection of 17 strains isolated from five different geographical locations (Mallorca, Alicante, Ebro Delta, Canary Islands, and Peruvian Andes) were studied following the currently accepted taxonomic approach. Additionally, random amplification of genomic DNA led to the phenetic analysis of the intraspecific diversity. Altogether the taxonomic study indicated that S. ruber remained highly homogeneous beyond any geographical barrier. However, genomic fingerprints indicated that populations from different isolation sites could still be discriminated.
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany.
A Gram-positive, non-spore-forming bacterium (GW39-1573(T)) was isolated from soil of the Spanish island of Tenerife. 16S rRNA gene sequence similarity studies showed that strain GW39-1573(T) belonged to the genus Nocardia and was most closely related to Nocardia brasiliensis (98.0 %), Nocardia beijingensis (97.3 %), Nocardia transvalensis (97.5 %), Nocardia asteroides (97.2 %) and Nocardia farcinica (97.0 %). Strain GW39-1573(T) could be distinguished from all other validly described Nocardia species by sequence similarity values of less than 97 %. Chemotaxonomic data [major menaquinone: MK-8(H(4, omega-cycl)); major polar lipids: diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and an unknown glycolipid and an unknown phospholipid; major fatty acids: C(16 : 0), C(18 : 1)omega9c and 10 methyl C(16 : 0)] and the presence of mycolic acids supported the affiliation of strain GW39-1573(T) to the genus NOCARDIA: The results of DNA-DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain GW39-1573(T) from those related species that showed 16S rRNA gene sequence similarity values of greater than 97 %. Strain GW39-1573(T) merits species status, and the name Nocardia tenerifensis is proposed with the type strain GW39-1573(T)(=DSM 44704(T)=CIP 107929(T)).
Hans-Jürgen Busse,
Christian Zlamala,
Sandra Buczolits,
Werner Lubitz,
Peter Kämpfer,
Mariko Takeuchi
Institut für Mikrobiologie und Genetik, Universität Wien, A-1030 Wien, Austria. hans-juergen.busse@vu-wien.ac.at
Two airborne bacterial isolates designated V45(T) and V54A(T) were characterized in order to determine their taxonomic position. 16S rDNA sequence analysis showed that the two isolates shared 98.1 % sequence similarity. Highest sequence similarities (98.0-98.5 %) were found to Promicromonospora citrea DSM 43110(T) and Promicromonospora sukumoe IFO 14650(T). Detection of a quinone system with the predominant compound MK-9(H(4)), a polar lipid pattern containing phosphatidylglycerol, a fatty acid profile with the predominant acids C(15 : 0) iso and C(15 : 0) anteiso and the diagnostic cell-wall diamino acid L-lysine supported the assignment of the novel isolates to the genus PROMICROMONOSPORA: The two isolates could be distinguished from P. sukumoe by the presence of glycine in the peptidoglycan, and the detection of the cell-wall sugar galactose differentiates them from the two established species of the genus PROMICROMONOSPORA: Each of the two isolates displayed a unique biochemical profile. Results from DNA-DNA hybridizations clearly demonstrated that V45(T) and V54A(T) represent separate species. Based on these data, it is proposed that V45(T)(=IFO 16525(T)=CCM 7044(T)) and V54A(T)(=IFO 16526(T)=CCM 7043(T)) be classified as the type strains of two novel Promicromonospora species, for which the names Promicromonospora vindobonensis sp. nov. and Promicromonospora aerolata sp. nov. are proposed.
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany. peter.kaempfer@agrar.uni-giessen.de
A format for the description of single novel species is proposed, which should facilitate the reviewing process by assisting the provision of data in a standardized form. The abstract must be short and concise, highlighting phylogenetic position, morphology and chemotaxonomy for genus affiliation, the genotypic and phenotypic basis for species differentiation, and the name and deposition numbers from two public culture collections in different countries for the type strain: A Gram-negative, rod-shaped, non-spore-forming bacterium (Iso 196(T)) was isolated from chicken faeces. On the basis of 16S rRNA gene sequence similarity, strain Iso 196(T) was shown to belong to the alpha-2 subclass of the Proteobacteria related to Ochrobactrum tritici (95.6%), Ochrobactrum grignonense (95.0%) and Ochrobactrum anthropi (94.6%), and the phylogenetic distance from any validly described species within the genus Brucella was less than 95%. Chemotaxonomic data (major ubiquinone - Q-10; major polyamines - spermidine and putrescine; major polar lipids - phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine; major fatty acids - C(18 : 1)omega7c and C(19 : 0) cyclo omega8c) supported the affiliation of strain Iso 196(T) to the genus Ochrobactrum. The results of DNA-DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain Iso 196(T) from the four validly published Ochrobactrum species. Iso 196(T) therefore represents a new species, for which the name Ochrobactrum gallinifaecis sp. nov. is proposed, with the type strain Iso 196(T)(= DSM 15295(T)= CIP 107753(T)).
Latest similar papers:
Luciane A Chimetto,
Ilse Cleenwerck,
Cristiane C Thompson,
Marcelo Brocchi,
Anne Willems,
Paul de Vos,
Fabiano L Thompson
Federal University of Rio de Janeiro, Brazil;
Four novel isolates (R-40508T, R-40507, R-40903 and R-21419) were obtained from different cnidarian species (Phyllogorgia dilatata, Merulina ampliata, and Palythoa caribaeorum) from different places in Brazil and Australia. The novel isolates formed a tight phylogenetic group based on 16S rRNA, recA, topA, ftsZ, mreB and rpoA. Their closest phylogenetic neighbours were Photobacterium leiognathi, P. rosenbergii and P. halotolerans, sharing 97.1 to 97.5 % 16S rRNA gene sequence similarity. DNA-DNA hybridization (DDH) between a representative strain (R-40508T) and the type strains of these Photobacterium species revealed less than 20 % DDH similarity, proving that the novel isolates belong to a new species. Several phenotypic features allow the differentiation of the new species from its closest phylogenetic neighbours. It has gelatinase and lipase activity, and can utilize melibiose, but it cannot grow on 6 % NaCl. In addition, the novel species has the fatty acid C16:0 iso, but it lacks the fatty acids C17:0, C17:0 cyclo, C17:0 iso, C17:1 omega8c, and C17:1 omega9c iso. The name Photobacterium jeanii sp. nov. is proposed for this taxon. The G+C content of the type strain R-40508T (= LMG 25436T = CAIM 1817T) is 45.5 mol%.
Finnish Forest Research Institute;
Five cold adapted bacteria belonging to the genus Mucilaginibacter were isolated from lichen and soil samples collected from Finnish Lapland and investigated in detail by phenotypic and phylogenetic analyses. Based on 16S rRNA gene phylogeny the new strains represent three new branches within Mucilaginibacter. The strains were aerobic chemoorganotrophic, non-motile rods and formed pigmented, smooth, mucoid colonies on solid media. The strains grew between 0 and 33 degrees C (with an optimum at 25 degrees C) and within pH range 4.5-8 (with an optimum at pH 6). The main cellular fatty acids were, iso-C15:0, iso-C15:0 2-OH, C16:1omega7c and iso-C17:0 3-OH and the major respiratory quinone was MK-7. The G+C content of DNA was 44-46 %. Based on the phylogenetic, phenotypic and chemotaxonomic data the strains represent three novel species of the genus Mucilaginibacter for which the names M. frigoritolerans (Type strain FT22T = ATCC BAA-1854 = LMG 25359) M. lappiensis (Type strain ANJLI2T = ATCC BAA-1855 = LMG 25358) and M. mallensis (Type strain MP1X4T = ATCC 1856 = LMG 25360) are proposed.
1 Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Life Science, Southwest University, Chongqing 400715, China.
Four species of the coelotine spider genus Himalcoelotes Wang, 2002 endemic to the Himalayas were studied: H. gyirongensis ( Hu and Li, 1987 ); H. tortuous sp. nov.; H. xizangensis ( Hu, 1992 ); and H. zhamensis sp. nov. The male of H. xizangensis is here described for the first time. A key to all 13 Himalcoelotes species is given. Twelve Himalcoelotes species are divided into two species groups. The species-group placement of H. aequoreus Wang, 2002 is uncertain.
Biology Centre of the Academy of Sciences CR, v.v.i. Institute of Hydrobiology;
Two bacterial strains II-B4(T) and II-D5(T), isolated from the meso-eutrophic freshwater Rimov Reservoir (Czech Republic) were phenotypically, phylogenetically and chemotaxonomically characterized. Both strains are chemoorganotrophic, facultatively anaerobic, rod-shaped, non-motile with identical G+C contents of their DNA of 59.9 mol%. Their major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and their major fatty acids were C(16:1)omega7c/C(16:1)omega6c, C(16:0), C(18:1)omega7c/C(18:1)omega6c and C(12:0). Both strains contained Q-8 as the only respiratory quinone component. The 16SrRNA sequences of the two strains posses a similarity of 99.1%, however the DNA-DNA reassociation value was only 26.7%, and the strains can be discriminated from each other by several chemotaxonomic and biochemical traits. The phylogenetic analysis of the 16S rRNA gene sequences revealed the affiliation of both strains with the genus Limnohabitans within the family Comamonadaceae. The two investigated strains represent the first isolated members of a narrow phylogenetic cluster (the so-called R-BT065 cluster) formed by a large number of environmental sequences and abundant populations detected in the pelagic zones of various freshwater habitats. We propose to place the two strains in separate new species within the genus Limnohabitans. The two proposed species are Limnohabitans planktonicus sp. nov. with the type strain II-D5(T)(= DSM 21594(T)= CIP 109844(T)), and Limnohabitans parvus sp. nov. with the type strain II-B4(T)(= DSM 21592(T)= CIP 109845(T)).The description of the genus Limnohabitans is emended accordingly.
Japan Biological Informatics Consortium (JBIC);
Three Gram-positive, sea salt requiring actinobacteria were isolated from a marine sponge Haliclona sp. collected from the coast of Tateyama city, Japan. A 16S rRNA gene sequence comparison indicated that these strains represent novel members of the genus Streptomyces, exhibiting low 16S rRNA gene sequence similarities of 98.3-97.4% with the validly published members of the genus. The cell hydrolysates contain LL-isomer of DAP and the predominant quinones were MK-9 (H6 and/or H8). The G + C content were in a range of 72-75 mol%. Polyphasic phylogenetic study of the strains and comparison of the characters with previously published related members of the genus show that these strains represent three new species of the genus Streptomyces. Therefore, the names S. tateyamensis sp. nov., S. haliclonae sp. nov., and S. marinus sp. nov., are proposed for strains Sp080513SC-30T (=NBRC 105048T = DSM 41969T), Sp080513SC-31T (= NBRC 105049T = DSM 41970T) and Sp080513GE-26T (= NBRC 105047T = DSM 41968T), respectively.
Institute for Food Safety and Hygiene, University of Zurich;
Five isolates, Gram-negative, facultative anaerobic, non-spore-forming coccoid rods, were obtained from infant formula and infant formula production environment and investigated in a polyphasic taxonomic study. Biochemical tests and partial rpoB gene sequence analysis of the five isolates revealed that they formed two distinct groups in the family Enterobacteriaceae, closely related with several Pantoea spp. and Erwinia spp, which indicated a phylogenetic position in the genus Pantoea or the genus Erwinia. Multilocus sequence analysis of concatenated partial atpD, gyrB, infB and rpoB gene sequences of two representatives, suggested that they constituted probably two novel species in the genus Pantoea, phylogenetically closely related to Pantoea septica. The five isolates showed the general characteristics of the genus Pantoea, and DNA-DNA hybridizations between two representatives and the type strains of the phylogenetically closest relatives according to comparative 16S rRNA sequence analysis, showed that the isolates represented two novel genospecies. These two genospecies could be differentiated from each other by fermentation of galactonurate, sorbitol and potassium-5-ketogluconate. From the phylogenetically related Pantoea species they could be distinguished by their ability to ferment D-lactose and utilize beta-gentiobiose and D-raffinose, their inability to ferment and utilize D-arabitol and their inability to produce indol.On the basis of the obtained results it is proposed to classify the isolates as Pantoea gaviniae, sp. nov.(type strain A18/07T = LMG 25382T = DSM 22758T) and Pantoea calida, sp. nov.(four isolates, type strain 1400/07T = LMG 25383T = DSM 22759T).
Hyungki Lee,
Seoung-Ae Lee,
In-Kyung Lee,
Hee-Kyung Yu,
Young-Gil Park,
Joseph Jeong,
Seon Ho Lee,
Sung-Ryul Kim,
Jin-Won Hyun,
Kijeong Kim,
Yoon-Hoh Kook,
Bum-Joon Kim
Department of Microbiology and Immunology, Cancer Research Institute, Liver Research Institute and SNUMRC, College of Medicine, Seoul National University, Seoul 110-799.
A previously unidentified, slowly growing scotochromogenic Mycobacterium was isolated from a Korean patient with symptomatic pulmonary infection. Phenotypically, this strain was generally similar to Mycobacterium terrae complex strains, however it uniquely produced orange pigmentation. Unique mycolic acid profiles and phylogenetic analyses based on three alternative chronometer molecules, 16S rRNA gene, hsp65 and rpoB, confirmed the taxonomic status of this strain as a novel species. These results support that this strain represents a novel Mycobacterium species. The name Mycobacterium paraterrae sp. nov. is proposed. The type strain is 05-2522 (= DSM 45127 = KCTC 19556).
Hang-Yeon Weon,
Soon-Wo Kwon,
Jung-A Son,
Eun-Hye Jo,
Soo-Jin Kim,
Yi-Seul Kim,
Byung-Yong Kim,
Jong-Ok Ka
National Academy of Agricultural Science;
Two bacterial strains 5420S-12T and 5420S-16T isolated from air samples, were characterized using a polyphasic approach. 16S rRNA gene sequence analysis showed that strain 5420S-12T was phylogenetically related to Microvirga subterranea Fail4T (97.3 % sequence similarity) and Microvirga guangxiensis 25BT (97.1 % similarity), and strain 5420S-16T was closely related to Balneimonas flocculans TFBT (98.2 % sequence similarity) and Microvirga guangxiensis 25BT (97.2 % similarity). The G+C content of the genomic DNA was 62.2 mol% for strain 5420S-12T and 61.5 mol% for strain 5420S-16T. The major fatty acid was C18:1omega7c. The results of DNA-DNA hybridization and phenotypic data showed that strains 5420S-12T and 5420S-16T could be distinguished from their phylogenetically related species, and represent two novel species within the genus Microvirga, for which the names Microvirga aerophila sp. nov.(type strain 5420S-12T = KACC 11743T = NBRC 106136T) and Microvirga aerilata sp. nov.(type strain 5420S-16T = KACC 11744T = NBRC 106137T) are proposed. Furthermore, the reclassification of Balneimonas flocculans as Microvirga flocculans comb. nov. is proposed and also emended description of the genus Microvirga is provided.
Gerard J M Verkley,
Josepa Gené,
Josep Guarro,
Juan J Pérez-Santonja,
Alejandra E Rodríguez,
M Francisca Colom,
Jorge L Alio,
Consuelo Ferrer
CBS Fungal Biodiversity Centre, Utrecht, The Netherlands.
The new coelomycete Pyrenochaeta keratinophila, isolated from corneal scrapings of a case of keratitis in Spain, is described and illustrated. This fungus is morphologically characterized by grey-olivaceous to greenish olivaceous colonies, scarce pycnidial setae placed mainly near the ostiole and production of phialoconidia from the aerial mycelium. The latter feature is unknown in any other species of the genus Pyrenochaeta. Sequencing of the ITS rDNA region of this clinical strain confirmed this proposal and revealed its close genetic relationship with the Leptosphaeriaceae.