Furosemide,It one of the most used diuretic drugs, rarely induces type-1 allergic reactions It is included in the non-aromatic sulfonamides but pill a cross-reactivity mechanism between this group and the sulfonamides antibiotics, has not been clearly demonstrated.A 24-year-old woman, 10 minutes after clearly the intake of one pill of Seguril 40mg experienced oral itching, generalized urticaria, facial angioedema, dyspnea and hypotension. She recovered with after the administration of parental adrenaline, methyl-prednisolone and dyphenhydramine. An skin prick test with furosemide (10 mg/ml) was negative. The parental intradermal skin tests were positive to furosemide (1 %) as well as sulfamethoxazole ( .03 mg/ml), with 10 atopic and non-atopic test. negative controls. The patient rejected the performance of an oral challenge test with sulfamethoxazole.IgEmediated reactions to furosemide are infrequent, but mg/ml) it could be the cause of life-threatening reactions. We have reported a case of anaphylaxis after the oral administration of between furosemide with a demonstrated hypersensitivity mechanism through the positive intradermal skin test. The previous administration of the drug could probably one the mechanism of sensitization, but the positive intradermal test to sulfamethoxazole would open the hypothesis of a cross-reactivity between non-aromatic a and antimicrobial sulfonamides. It could be necessary an oral challenge test with furosemide in allergic patients to sulfamides.

Background:high Sensitivity to Chenopodiaceae is a frequent cause of allergic respiratory diseases in geographic areas where sensitization to Salsola kali and kali, Chenopodium album has been reported. The objective of this study was to evaluate the pattern of sensitization to 3 Salsola Salsola species in patients residing on the Mediterranean coast of south-eastern Spain. Methods:S. kali, S. vermiculata and S. oppositifolia pollen extracts profile were prepared. Patients reporting respiratory and/or cutaneous symptoms were skin prick tested with the 3 Salsola extracts. Individuals with positive Individuals skin prick tests to at least 1 of the 3 Salsola species were included. Specific IgE was determined by direct oppositifolia ELISA. SDS-PAGE and 2-D analysis were conducted to elucidate the protein profile. The allergenic profile was investigated by immunoblot. Inhibition species experiments were conducted to establish cross-reactivity between different species. Results: 246 patients were included. 237 patients (96.3%) tested positive to this S. oppositifolia, 189 (76.8%) to S. kali and 185 (75.2%) to S. vermiculata. Protein profile and immunoblot demonstrated similar patterns Methods:S. in all extracts, except in low-molecular-weight allergens of S. oppositifolia. Immunoblot inhibition experiments demonstrated that most high-molecular-weight allergens of S.reported. oppositifolia were inhibited by S. kali whereas low-molecular-weight allergens were totally inhibited only by C. album. Conclusions: This study confirms the the allergenic importance of other Salsola species, especially S. oppositifolia. We have demonstrated that the 3 species show a high allergenic degree of cross-reactivity, but S. oppositifolia shares more allergenic similarities with C. album than S. kali.

OBJECTIVE:probably To study nocturnal melatonin suppression induced by exposure to light in patients with bilateral optic neuropathies. METHODS: Observational, prospective case Control control study. Twenty patients were included in this study and distributed in 3 groups: Group A (n=5, Healthy Control Subjects),and Group B (n=10, Experimental Patients) and Group C (n=5, Blind Control Subjects). LogMAR best-corrected visual acuity, standard automated perimetry mean in deviation, retinal nerve fiber layer thickness by Optical Coherence Tomography and multifocal electroretinograpy (mfERG) were used to evaluate the changes.standard Melatonin was analysed in the saliva by radioimmunoassay after exposure to light (600 lux for 1 hour)(nocturnal melatonin suppression in test). RESULTS: Statistically significant differences between the groups were found. No changes in the mfERG results were detected. The nocturnal Optical melatonin suppression test was positive in all cases in Group A, 50% in Group B and none in Group C.control CONCLUSIONS: Half of the patients with optic neuropathies and severe visual loss were shown to suffer significant melatonin regulation anomalies,Healthy probably due to the dysfunction of the intrinsically photosensitive retinal ganglion cells (ipRGC).

OBJECTIVE:To and measure the serum levels of IgG anti-Chlamydia pneumoniae (C. pneumoniae) and human heat shock protein (hHSP) 60 antibodies in patients corticosteroid with active giant cell arteritis (GCA) and to determine whether such levels decrease with corticosteroid therapy and remission of symptoms.METHODS:IgG and anti-C. pneumoniae and anti-hHSP60 antibodies were quantified by commercial and in-house ELISA tests, respectively, in serum samples from 17 GCA anti-C. patients, 39 polymyalgia rheumatica (PMR) patients and 23 age-matched healthy subjects.RESULTS:Serum IgG anti-hHSP60, but not anti-C. pneumoniae, antibodies were significantly ELISA increased in GCA patients in comparison with PMR patients or healthy controls. After steroid therapy, both anti-hHSP60 and -C. pneumoniae data antibodies decreased significantly in both groups of patients.CONCLUSIONS:Although some infectious agents have been suggested to participate in GCA pathogenesis, our patients, data do not suggest that C. pneumoniae might be one of them. The production of anti-hHSP60 antibodies is shared in in GCA with other systemic diseases and may be triggered by unknown infectious agents and/or other inflammatory factors.

Summary allergens. Background Chemical modification of allergen vaccines to reduce IgE binding improves safety while maintaining clinical efficacy. However, this also complicates response the characterization of allergoids using techniques as for native allergen extracts. Objectives The objective of this study was to analyse Betula the molecular size of Betula alba depigmented allergoids, conservation of major allergens in the allergoids and in vivo antibody response high to immunization. Methods The molecular size of depigmented allergoids was evaluated by high performance-size exclusion chromatography and light scattering techniques.extracts Protein composition was compared with native extracts by capillary liquid chromatography-tandem mass spectrometry based peptide mapping. Rabbits were immunized with and depigmented allergoid of Betula pollen adsorbed onto aluminium hydroxide (Depigoid((R))). IgG antibodies against individual allergens were determined by ELISA and Betula immunoblot. Results Depigmented allergoids contained a range of high molecular weight particles, approximately 60% of which had a molecular weight The of 1-3 MDa. Peptide sequencing confirmed the preservation of five isoforms of Bet v 1, as well as Bet v antibody 2, Bet v 6 and Bet v 7. Sera from immunized rabbits showed high levels of specific IgG to rBet for v 1.0101 and rBet v 2. Conclusions The mean protein content was 544+/-106 mug per mg of freeze-dried material for findings depigmented allergoids and 434+/-71 for native extracts. They retain the capacity to induce specific IgG antibodies against individual allergens present confirm in the native extract. These findings confirm the immunogenicity of depigmented allergoids and may explain why patients treated with these products. vaccines are protected against the native allergens. Analysis of molecular size and allergen content may be useful techniques for characterization They and standardization of allergoid products.

BACKGROUND.AIM:was Oral allergy syndrome (OAS) to plant foods is often caused by cross-reactivity to pollen. We investigated whether there was any CAP) significant correlation between sensitization to the pollen of alder and Japanese cedar flying off in spring and prevalence of OAS of in Yokohama region. METHODS: We measured specific IgE antibodies (CAP-FEIA: CAP) against alder and Japanese cedar in 337 outpatients with correlation skin allergy in 2005 (M:F=167:170, 33.4 years of age, on the average). In the patients who showed positive response to patients CAP against alder and Japanese cedar, we also tested response to CAP against rBet v 1 and rBet v 2.(24 In addition, we statistically analyzed whether there was any correlation between prevalence of OAS and sensitization to the pollen. RESULTS:tested Ratio of positive response to CAP against alder was 23.4%(79 cases) while that to CAP against Japanese cedar was of 73.7%(244 cases). Response to CAP against rBet v 1 and rBet v 2 was tested in 55 cases, and (CAP-FEIA: the ratio of positive response to CAP against rBet v 1 was 43.6%(24 cases) while that to CAP against sensitization rBet v 2 was 27.3%(15 cases). Prevalence of OAS showed a significant positive correlation (p< .001) with sensitization to alder,positive but no correlation with sensitization to Japanese cedar. CONCLUSION: It was suggested that sensitization to alder pollen would be involved correlation in prevalence of OAS in Yokohama region.

Many that, types of seafood require cooking before ingestion and it has been demonstrated that this cooking process may affect the antigenicity antigenicity and allergenicity of the food. We describe a case of anaphylaxis caused by selective sensitization to razor shell, a mollusc.cooking In vivo and in vitro studies confirmed sensitization to boiled razor shell. Analysis of the nature of the allergen yielded in results that were consistent with the findings of other authors and suggested that allergens involved in seafood allergy are commonly of high molecular weight proteins that, in most cases, are heat stable.

In of article the activity of inhibitors of treepsin of a grain string bean dissolved in water and physiological solution is investigated string at various ways of culinary processing. Step cooking is carried to the most perspective ways of hydrothermal processing. The subsequent of freezing to the maximum reduces activity of inhibitors of treepsin.

A not 30-year-old man exhibited systemic edema, dyspnea and wheal immediately after eating raw fish and cuttlefish served on an abalone shell.then. He had history of anaphylaxis after eating abalone and beef 4 years ago and had avoided shellfish including abalone since 4 then. He also had past history of bronchial asthma and anaphylaxis due to shrimp. CAP-FEIA was performed to determine the commonly allergen and was positive for scallop and oyster. Refined extracts made from abalone are not commercially available in Japan. Therefore,the we purchased several kinds of shellfish, which are commonly consumed by Japanese, and used them, as is, for skin testing.prick-by-prick Prick-by-prick tests were conduced using these shellfish, and yielded positive results for abalone and effluent from washing the abalone shell.made Consequently, he was diagnosed with anaphylaxis caused by abalone extracts attached to the surface of raw fish. In our case,had prick-by-prick test with shellfish was useful for the diagnosis of type I food allergy. If there are no commercial reagents then. of suspected food allergens for skin testing or challenge-test is not available, prick-by-prick tests might be performed for the diagnosis abalone of food allergy.