dvmphd
| name & location | Mark A. Kukucka, MS, DVM, PhD @ Kingsville, MD, United States // (male) |
| disciplines | Biochemistry, Cell biology, Nanotechnology, Veterinary medicine |
| affiliation |
Virginia Tech, United States, VA Blacksburg, 24060 http://www.vetmed.vt.edu/ |
| groups | all @ Virginia Tech; Free Radicals; Testosterone; Ascorbic Acid; Oxytocin; Leydig Cells; |
| educational background | |
| 1999-11 - 1999-11 |
Degree: Doctoral @ Virginia Tech, Blacksburg VA, United States Mechanisms by which hypoxia augments Leydig cell viability and differentiated cell function in vitro http://scholar.lib.vt.edu/theses/available/etd-06062008-170416 |
| skills | |
| expert | Wikipedian Editor - http://en.wikipedia.org/wiki/user:dvmphd |
| awards | |
| 2008-12 | Master Spaatz Award # 569 --- http://www.spaatz.org/mbr/list.php |
| latest report |
A specific high-performance liquid chromatographic (HPLC) method is described for the reliable quantitation of oxytocin using culture media supernatants. The procedure employs solid-phase extraction, antibody-based immunoaffinity purification and isocratic HPLC with dual channel coulometric detection (ED). The lower limit of detection for this cyclic nonapeptide was 40 pg (40 fmol). Due to its relative simplicity, specificity and precision, the HPLC-ED of oxytocin is an accurate and attractive alternative to many existing quantitative methods.